Abstract
A prerequisite for an antigenically defined vaccine against malaria is the identification of parasite antigens which are necessary or sufficient for the induction and expression of host-protective immunity. As malaria infection in man can be treated by passive transfer of serum or immunoglobulin from adults in an endemic area1 it is reasonable to propose that sera from clinically defined patient groups and functionally defined immunoglobulin may be used as probes to identify the relevant parasite antigens involved in host protection (‘host-protective antigens’)2 We have now purified immunoglobulin from a large number of individuals living in the endemic area, tested for inhibitory effects on in vitro growth of Plasmodium falciparum originating from the same area, then examined antibody specificities by immunoprecipitation analysis with biosyntheti-cally labelled proteins of blood stages of the parasite. Two-dimensional gel analysis of immunoprecipitates revealed a correlation between inhibition of parasite growth and increased antibody binding to two proteins of molecular weight (Mr) ∼96,000.
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Brown, G., Anders, R., Mitchell, G. et al. Target antigens of purified human immunoglobulins which inhibit growth of Plasmodium falciparum in vitro. Nature 297, 591–593 (1982). https://doi.org/10.1038/297591a0
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DOI: https://doi.org/10.1038/297591a0
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