Abstract
Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as two overlapping spliced mRNAs, 12S and 13S, which encode closely related proteins. To distinguish between the functions of these proteins, a single T → G transversion was constructed which prevents splicing of the 12S mRNA. This transversion, in the second base of the 12S mRNA intron, does not alter the protein encoded by the 13S mRNA due to degeneracy in the genetic code. Studies with this mutant demonstrated that only the 13S mRNA encodes the regulatory protein required for normal early gene expression.
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References
Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. Natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).
Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin, S. L. & Steitz, J. A. Nature 283, 220–224 (1980).
Rogers, J. & Wall, R. Proc. Natn. Acad. Sci. U.S.A. 77, 1877–1879 (1980).
Berk, A. J. & Sharp, P. A. Cell 14, 695–711 (1978).
Berk, A. J., Lee, F., Harrison, T., Williams, J. & Sharp, P. A. Cell 17, 935–944 (1979).
Jones, N. & Shenk, T. Proc. Natn. Acad. Sci. U.S.A. 76, 3665–3669 (1979).
Graham, F. L., Harrison, T. & Williams, J. Virology 86, 10–21 (1978).
Jones, N. & Shenk, T. Cell 17, 683–689 (1979).
Perricaudet, M., Akusjarvi, G., Virtanen, A. & Petterson, U. Nature 281, 694–696 (1979).
Baker, C. C. & Ziff, E. B. J. Molec. Biol. 149, 189–221 (1981).
Harrison, T., Graham, F. & Williams, J. Virology 77, 319–329 (1977).
Ricciardi, R. P., Jones, R. L., Cepko, C. L., Sharp, P. A. & Roberts, B. E. Proc. Natn. Acad. Sci. U.S.A. 78, 6121–6125 (1981).
Razin, A., Hirose, T., Itakura, K. & Riggs, A. Proc. Natn. Acad. Sci. U.S.A. 75, 4268–4270 (1978).
Hutchison, C. A. et al. J. biol. Chem. 253, 6551–6560 (1978).
Gillam, S., Astell, C. R. & Smith, M. Gene 12, 129–137 (1980).
Matteucci, M. D. & Caruthers, M. H. J. Am. chem. Soc. 103, 3185–3191 (1981).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Smith, A. J. H. Meth. Enzym. 65, 560–580 (1980).
Stow, N. D. J. Virol. 37, 171–180 (1981).
Graham, F. L., Smiley, J., Russell, W. C. & Nairn, R. J. gen. Virol. 36, 59–72 (1977).
Aiello, L., Huebner, K. & Weinmann, R. Virology 94, 460–469 (1979).
Lassam, N. J., Bayley, S. T. & Graham, F. L. Cold Spring Harb. Symp. Quant. Biol. 44, 477–491 (1980).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Sambrook, J. et al. Cold Spring Harb. Symp. Quant. Biol. 44, 569–584 (1980).
Spector, D. J., McGrogan, M. & Raskas, H. J. J. Molec. Biol. 126, 395–414 (1978).
Chow, L. T., Broker, T. R. & Lewis, J. B. J. Molec. Biol. 134, 265–303 (1979).
Frost, E. & Williams, J. Virology 91, 39–50 (1978).
Solnick, D. Nature 291, 508–510 (1981).
Baird, M. et al. Proc. natn. Acad. Sci. U.S.A. 78, 4218–4221 (1981).
Shander, M., Woude, S. V., Proudfoot, N. & Maniatis, T. J. supramolec. Struct. Cell Biochem. (Suppl.) 5, 229 (1981).
Ohshima, Y., Itoh, M., Okada, N. & Miyata, T. Proc. natn. Acad. Sci. U.S.A. 78, 4471–4474 (1981).
Yang, V. W., Lerner, M. R., Steitz, J. A. & Flint, S. J. Proc. natn. Acad. Sci. U.S.A. 78, 1371–1375 (1981).
Peebles, C. L., Ogden, R. C., Knapp, G. & Abelson, J. Cell 18, 27–37 (1979).
Dijkema, R. et al. Gene 12, 287–299 (1980).
Sawada, Y. & Fujinaga, K. J. Virol. 36, 639–651 (1980).
van Ormondt, H., Maat, J., De Waard, A. & van der Eb, A. J. Gene 4, 309–328 (1978).
Osborne, T. F., Schell, R. E., Burch-Jaffe, E., Berget, S. J. & Berk, A. J. Proc. natn. Acad. Sci. U.S.A. 78, 1381–1385 (1981).
Hines, J. C. & Ray, D. S. Gene 11, 207–218 (1980).
Messing, J. Recombinant DNA tech. Bull. 2, 43–48 (1979).
Jay, E., Bambara, R., Padmanabham, R. & Wu, R. Nucleic Acids Res. 1, 331–353 (1973).
Bolivar, F. et al. Gene 2, 95–113 (1977).
van der Eb, A. J. & Graham, F. L. Meth. Enzym. 65, 826–839 (1980).
Anderson, S. Nucleic Acids Res. 9, 3015–3027 (1981).
Dunsworth-Browne, M., Schell, R. E. & Berk, A. J. Nucleic Acids Res. 8, 543–554 (1980).
Nomura, N. & Ray, D. A. Proc. Natn. Acad. Sci. U.S.A. 77, 6566–6570 (1980).
Vogt, V. M. Eur. J. Biochem. 33, 192–200 (1973).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
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Montell, C., Fisher, E., Caruthers, M. et al. Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature 295, 380–384 (1982). https://doi.org/10.1038/295380a0
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DOI: https://doi.org/10.1038/295380a0
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