Abstract
Calcitonin is a small peptide hormone (molecular weight (MW) 3,500) synthesized and secreted in mammals by the C-cells of the thyroid1,2. In man a major role of calcitonin is to protect the skeleton during periods of calcium stress such as growth, pregnancy and lactation3. Excessive calcitonin levels are associated with familial and sporadic medullary carcinoma of the thyroid4, and in some cases with ectopic synthesis often by small oat-cell carcinoma of the lung5. Evidence from mRNA-directed cell-free protein synthesis suggests that in common with other small peptide hormones6, human calcitonin is synthesized as a high-molecular-weight precursor protein7–9. Recently, we described the construction and partial characterization of recombinant plasmids containing human calcitonin cDNA sequences9. Here we demonstrate by nucleotide sequence analysis of these cloned cDNA sequences that human calcitonin resides towards the carboxy terminal of a precursor polyprotein, and is flanked at the amino and carboxy termini by cryptic peptide sequences of unknown biological significance. The cryptic peptide present at the carboxy terminus differs significantly in amino acid sequence from that present in the rat calcitonin precursor polyprotein10–12.
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References
Foster, G. V., MacIntyre, I. & Pearse, A. G. E. Nature 203, 1029–1030 (1964).
Foster, G. V. et al. Nature 702, 1303–1305 (1964).
Stevenson, J. C., Hillyard, C. J., MacIntyre, I., Cooper, H. & Whitehead, M. I. Lancet ii, 769–770 (1979).
Stevenson, J. C. & Hillyard, C. J. in Recent Results in Cancer Research (ed. Duncan, W.) 60–67 (Springer, Heidelberg, 1980).
Schwartz, K. E., Wolfsen, A. R., Forster, B. & Odell, W. D. J. clin. Endocr. Metab. 49, 438–444 (1979).
Herbert, E. Trends biochem. Sci. 6, 184–188 (1981).
Desplan, C. et al. FEBS Lett. 117, 89–92 (1980).
Goodman, R. H., Jacobs, J. W. & Habener, J. F. Biochem. biophys. Res. Commun. 91, 932–938 (1979).
Allison, J., Hall, L., MacIntyre, I. & Craig, R. K. Biochem. J. 199, 725–731 (1981).
Amara, S. G., David, D. N., Rosenfeld, M. G., Roos, B. A. & Evans, R. M. Proc. natn. Acad. Sci. U.S.A. 77, 4444–4448 (1980).
Jacobs, J. W. et al. Science 213, 457–459 (1981).
Jacobs, J. W., Potts, J. T., Bell, N. H. & Habener, J. F. in Calcitonin 1980 (ed. Pecile, A.) 25–34 (Excerpta Medica, Amsterdam, 1981).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Riniker, B. et al. Helv. chim. Acta 51, 1738–42 (1968).
Neher, R., Riniker, B., Rittel, W. & Zuber, H. Helv. chim. Acta 51, 1900–1905 (1968).
Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H. S. Ann. N. Y. Acad. Sci. 343, 1–16 (1980).
Kreil, G., Suchanek, G. & Kindas-Mugge, I. Fedn. Proc. 36, 2081–2086 (1977).
Chang, A. C. Y., Cochet, M. & Cohen, S. N. Proc. natn. Acad. Sci. U.S.A. 77, 4890–4894 (1980).
Seidah, N. G., Rochmont, J., Hamelin, J., Lis, M. & Chretien, M. J. biol. Chem. 256, 7977–7984 (1981).
Proudfoot, N. J. & Brownlee, G. G. Nature 252, 359–362 (1974).
Jeffreys, A. J. in Genetic Engineering Vol. 2 (ed. Williamson, R.) 1–48 (Academic, New York, 1981).
Botstein, D., White, P. L., Skolnick, M. & Davis, R. W. Am. J. hum. Genet. 32, 314–331 (1980).
Stevenson, J. C. et al. Post-grad. med. J. 57, 104–108 (1981).
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Craig, R., Hall, L., Edbrooke, M. et al. Partial nucleotide sequence of human calcitonin precursor mRNA identifies flanking cryptic peptides. Nature 295, 345–347 (1982). https://doi.org/10.1038/295345a0
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DOI: https://doi.org/10.1038/295345a0
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