Abstract
An association between mitochondria and microtubules has been suggested from microscopic studies1,2 and by the cellular redistribution of mitochondria that is induced by colcemid, which disrupts microtubules1. Intermediate filaments have also been proposed to interact with mitochondria1. To investigate this association further, we have now studied the effect on the cytoskeleton of impairment of mitochondrial functions induced by three metabolic inhibitors: sodium azide, which inhibits electron transport; oligomycin, which inhibits ATP synthetase; and carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP), a proton ionophore which uncouples electron transport from ATP synthesis. We have found that treatment of cells with FCCP leads to a disruption of microtubules and an aggregation of vimentin filaments. This effect is not provoked by mitochondrial ATP depletion, but could be linked to another event related to the rapid dissipation of the mitochondrial electrochemical gradient.
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Maro, B., Bornens, M. Reorganization of HeLa cell cytoskeleton induced by an uncoupler of oxidative phosphorylation. Nature 295, 334–336 (1982). https://doi.org/10.1038/295334a0
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DOI: https://doi.org/10.1038/295334a0
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