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Soluble factor activation of human B lymphocytes

Nature volume 294, pages 261–263 (1981)Cite this article

Abstract

Medium conditioned by lectin (and/or antigen)-stimulated human peripheral blood lymphocytes has been shown to contain factors, termed interleukin 1 (IL-1) and interleukin 2 (IL-2)1,2, which augment and maintain human T-cell proliferation3–6, respectively. Interleukin 2 (T-cell growth factor, TCGF) requires the presence of a macrophage, or its monokine product, IL-1, for its production by T cells7–9. It has been shown that multiple signals are required for stimulating both T- and B-cell proliferation8–10 but the factors necessary for optimal B-cell mitogenesis have not been rigorously defined. As both B- and T-cell stimulatory agents are present in lectin-stimulated peripheral blood lymphocyte-conditioned media, we studied the effects of the interleukin factors on human B cells. Human peripheral blood B lymphocytes and pokeweed mitogen (PWM)-stimulated B-cell blasts were evaluated for their proliferative response to soluble growth factors, which were obtained from either lipopolysaccharide or phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells. We show here that factors from lipopolysaccharide-stimulated conditioned medium were unable to stimulate initial B-cell proliferation. However, factors from PHA-stimulated conditioned medium stimulated 3H-thymidine (TdR) incorporation in both B cells and lectin-activated T cells. This stimulation could be maintained for multiple re-exposures to the growth factors, which suggests that a soluble factor present in medium conditioned by lectin-stimulated peripheral blood mononuclear cells may modulate B-cell proliferation in a manner analogous to T-cell proliferation.

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Authors and Affiliations

  1. Department of Pathology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 6723 Bertner Avenue, Houston, Texas, 77030, USA

    Richard J. Ford, Shashikant R. Mehta, Daisy Franzini & Abby L. Maizel

  2. Associated Biomedic Systems, Buffalo, New York, 14202, USA

    Richard Montagna

  3. Department of Microbiology and Immunology, Duke University Medical Center, Box 3829, Durham, North Carolina, 27710, USA

    Lawrence B. Lachman

Authors
  1. Richard J. Ford
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  2. Shashikant R. Mehta
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  4. Richard Montagna
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  5. Lawrence B. Lachman
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  6. Abby L. Maizel
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Ford, R., Mehta, S., Franzini, D. et al. Soluble factor activation of human B lymphocytes. Nature 294, 261–263 (1981). https://doi.org/10.1038/294261a0

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