Insertion of DNA activates the cryptic bgl operon in E. coli K12

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Abstract

Spontaneous mutations which activate the cryptic bgl operon of Escherichia coli K12 are caused by insertion of DNA at a site, bglR, within the operon. Two insertion elements, IS1 and IS5, have been observed to effect this activation. Once the activating insertion has occurred the operon is inducible by βglucosides in a cyclic AMP-dependent manner.

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