Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Co-translational membrane integration of calcium pump protein without signal sequence cleavage

Nature volume 292, pages 87–88 (1981)Cite this article

Abstract

The calcium pump protein (CPP) or Ca2+-ATPase is the predominant integral membrane protein of the sarcoplasmic reticulum (SR) of skeletal muscle1,2. CPP is a single polypeptide chain of molecular weight (MW) 119,000 (ref. 3) which loops in and out of the membrane at least three times4–8. Recently, a number of integral transmembrane proteins possessing a single hydrophilic domain on each side of the membrane have been observed to use a signal sequence, cleaved9,10 or uncleaved11, to initiate translocation of their ectoplasmic domain12. Microsomal membranes when present during translation are capable of correctly integrating these de novo synthesized proteins into the membrane9,11. We have used this in vitro translocation system to investigate the integration of CPP into the microsomal membrane. We found that CPP is synthesized without a cleaved signal sequence and that it can be integrated into heterologous microsomal membranes only when these are present during translation, but not when they are present after completion of translation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. MacLennan, D. H. & Holland, P. C. A. Rev. Biophys. Bioengng. 4, 377–404 (1975).

    Article  CAS  Google Scholar 

  2. Fleischer, S., Wang, C.-T., Saito, A., Pilarska, M. & McIntyre, J. O. in Cation Flux Across Membranes (eds Mukohata, Y. & Packer, L.) 193–205 (Academic, New York, 1979).

    Book  Google Scholar 

  3. Rizzolo, L. J., le Maire, M., Reynolds, J. & Tanford, C. Biochemistry 15, 3433–3436 (1976).

    Article  CAS  Google Scholar 

  4. Thorley-Lawson, D. A. & Green, N. M. Eur. J. Biochem. 40, 403–413 (1973).

    Article  CAS  Google Scholar 

  5. Rizzolo, L. J. & Tanford, C. Biochemistry 17, 4044–4048 (1978).

    Article  CAS  Google Scholar 

  6. Saito, A., Wang, C.-T. & Fleischer, S. J. Cell Biol. 79, 601–616 (1978).

    Article  CAS  Google Scholar 

  7. Klip, A., Reithmeier, R. A. F. & Maclennan, D. H. J. biol Chem. 255, 6565–6568 (1980).

    Google Scholar 

  8. Allen, G., Trinnaman, B. J. & Green, N. M. Biochem. J. 187, 591–616 (1980).

    Article  CAS  Google Scholar 

  9. Lingappa, V. R., Katz, F. N., Lodish, H. F. & Blobel, G. J. biol. Chem. 253, 8667–8670 (1978).

    CAS  Google Scholar 

  10. Ploegh, H. L., Cannon, L. E. & Strominger, J. L. Proc. natn. Acad. Sci. USA. 76, 2273–2277 (1979).

    Article  CAS  ADS  Google Scholar 

  11. Bonatti, S. & Blobel, G. J. biol. Chem. 254, 12261–12264 (1979).

    CAS  PubMed  Google Scholar 

  12. Blobel, G. Proc. natn. Acad. Sci. U.S.A. 77, 1496–1500 (1980).

    Article  CAS  ADS  Google Scholar 

  13. Reithmeier, R. A. F., de Leon, S. & MacLennan, D. H. J. biol. Chem. 255, 11839–11846 (1980).

    CAS  PubMed  Google Scholar 

  14. Allen, G. Biochem. J. 187, 545–563 (1980).

    Article  CAS  Google Scholar 

  15. Lingappa, V. R., Devillers-Thiery, A. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 74, 2432–2436 (1977).

    Article  CAS  ADS  Google Scholar 

  16. Greenway, D. C. & MacLennan, D. Can. J. Biochem. 56, 452–456 (1978).

    Article  CAS  Google Scholar 

  17. Chyn, T. L., Martonosi, A. N., Morimoto, T. & Sabatini, D. D. Proc. natn. Acad. Sci. U.S.A. 76, 1241–1245 (1979).

    Article  CAS  ADS  Google Scholar 

  18. Vanderkooi, J. M., Ierokomas, A., Nakamura, H. & Martonosi, A. Biochemistry 16, 1262–1266 (1977).

    Article  CAS  Google Scholar 

  19. Wang, C.-T., Saito, A. & Fleischer, A. J. biol. Chem. 254, 9209–9219 (1979).

    CAS  PubMed  Google Scholar 

  20. le Maire, M., Møller, J. V. & Tanford, C. Biochemistry 15, 2336–2342 (1976).

    Article  CAS  Google Scholar 

  21. Steck, T. L. & Yu, J. J. supramolec. Struct. 1, 220–232 (1973).

    Article  CAS  Google Scholar 

  22. Shanahan, M. F. & Czech, M. P. J. biol. Chem. 252, 6554–6561 (1977).

    CAS  PubMed  Google Scholar 

  23. Neubig, R. R., Krodel, E. K., Boyd, N. D. & Cohen, J. B. Proc. natn. Acad. Sci. U.S.A. 76, 690–694 (1979).

    Article  CAS  ADS  Google Scholar 

  24. Walter, P., Jackson, R. C., Marcus, M., Lingappa, V. R. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 76, 1795–1799 (1979).

    Article  CAS  ADS  Google Scholar 

  25. Walter, P. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 77, 7112–7116 (1980).

    Article  CAS  ADS  Google Scholar 

  26. Anderson, D. J., Mostov, K. E., Walter, P. & Blobel, G. (in preparation).

  27. Mostov, K. E., DeFoor, P., Fleischer, S. & Blobel, G. Europ. J. Cell Biol. 22, 147 (1980).

    Google Scholar 

  28. DeFoor, P. H., Levitsky, D., Biryukova, T. & Fleischer, S. Archs Biochem. Biophys. 200, 196–205 (1980).

    Article  CAS  Google Scholar 

  29. Shields, D. & Blobel, G. Proc. natn. Acad. Sci. U.S.A. 74, 2059–2063 (1977).

    Article  CAS  ADS  Google Scholar 

  30. Schmidt, G. W., Devillers-Thiery, A., Desruisseaux, H., Blobel, G. & Chua, N.-H. J. Cell Biol. 83, 615–622 (1979).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Cell Biology, The Rockefeller University, New York, New York, 10021, USA

    Keith E. Mostov & Günter Blobel

  2. Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee, 37235, USA

    Paul DeFoor & Sidney Fleischer

Authors
  1. Keith E. Mostov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul DeFoor
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sidney Fleischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Günter Blobel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mostov, K., DeFoor, P., Fleischer, S. et al. Co-translational membrane integration of calcium pump protein without signal sequence cleavage. Nature 292, 87–88 (1981). https://doi.org/10.1038/292087a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/292087a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing