Abstract
Leghaemoglobin (Lb) has only been found in the nitrogen-fixing root nodules of legumes symbiotically associated with Rhizobia. Soybean nodules contain three major forms of Lb called a, c1 and c2, respectively. The amino acid sequences of the three Lbs have been determined recently1. Lb a differs from the two c varieties in six amino acids, while Lb c1 and c2 differ in only one amino acid. We have recently reported the isolation of a hybrid plasmid containing soybean Lb cDNA sequences2. Using one such clone as a probe, we have isolated five different soybean DNA fragments containing chromosomal Lb genes from a soybean DNA library which was constructed using lambda λgt Wes·λB as vector. The detailed analysis of one of the isolated chromosomal Lb genes revealed the presence of three intervening sequences. Alignment by homology of all known globin amino sequences shows that the splicing points of the first and the third intervening sequences in the Lb-coding sequence are located in the same positions as the two interruptions found in all other known globin-coding sequences.
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Jensen, E., Paludan, K., Hyldig-Nielsen, J. et al. The structure of a chromosomal leghaemoglobin gene from soybean. Nature 291, 677–679 (1981). https://doi.org/10.1038/291677a0
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DOI: https://doi.org/10.1038/291677a0
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