Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses

Nature volume 291, pages 675–677 (1981)Cite this article

Abstract

Three classes of avian sarcoma viruses have been defined recently (Table 1)1–5. Transformation-specific proteins of all three viral classes have associated tyrosine-specific protein kinase activity4–12. The protein kinase activity of Rous sarcoma viruses (RSV), representing class I, seems to be a property of the transformation-specific protein, pp60src, itself13 and seems to be essential for cellular transformation, as the activity is labile or absent in transformation mutants6,7,14,15. This connection between transformation-specific protein, enzyme activity and oncogenic cellular changes has yet to be established for the viruses of classes II and III, which code for polyproteins in which gag-derived and transformation-specific sequences are fused. We report here an analysis of the phosphorylation sites on the transformation-specific proteins encoded by RSV and the class III viruses which shows that, although there is no appreciable sequence relationship between large portions of these molecules, a tryptic peptide containing the phosphotyrosine seems to be identical in classes I and III.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Shibuya, M., Hanafusa, T., Hanafusa, H. & Stephenson, J. R. Proc. natn. Acad. Sci. U.S.A. 77, 6536–6540 (1980).

    Article  ADS  CAS  Google Scholar 

  2. Yoshida, M., Kawai, S. & Toyoshima, K. Nature 287, 653–654 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Neil, J. C., De Lamarter, J. & Vogt, P. K. Proc. natn. Acad. Sci. U.S.A. 78, 1906–1910 (1981).

    Article  ADS  CAS  Google Scholar 

  4. Beemon, K. Cell 24, 145–154 (1981).

    Article  CAS  PubMed  Google Scholar 

  5. Ghysdael, J., Neil, J. C. & Vogt, P. K. Proc. natn. Acad. Sci. U.S.A. 78, 2611–2615 (1981).

    Article  ADS  CAS  Google Scholar 

  6. Collett, M. S. & Erikson, R. L. Proc. natn. Acad. Sci. U.S.A. 75, 2021–2024 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Levinson, A. D., Oppermann, H., Levintow, L., Varmus, H. E. & Bishop, J. M. Cell 15, 561–572 (1978).

    Article  CAS  PubMed  Google Scholar 

  8. Hunter, T. & Sefton, B. M. Proc. natn. Acad. Sci. U.S.A. 77, 1311–1315 (1980).

    Article  ADS  CAS  Google Scholar 

  9. Kawai, S. et al. Proc. natn. Acad. Sci. U.S.A. 77, 6199–6203 (1980).

    Article  ADS  CAS  Google Scholar 

  10. Feldman, R., Hanafusa, T. & Hanafusa, H. Cell 22, 757–765 (1980).

    Article  CAS  PubMed  Google Scholar 

  11. Neil, J. C., Ghysdael, J. & Vogt, P. K. Virology 109, 223–228 (1981).

    Article  CAS  PubMed  Google Scholar 

  12. Ghysdael, J., Neil, J. C., Wallbank, A. M. & Vogt, P. K. Virology 111, 386–400 (1981).

    Article  CAS  PubMed  Google Scholar 

  13. Erikson, R. L., Collett, M. S., Erikson, E. & Purchio, A. F. Proc. natn. Acad. Sci. U.S.A. 12, 6200–6204 (1979).

    Google Scholar 

  14. Sefton, B. M., Hunter, T. & Beemon, K. J. Virol. 33, 220–229 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Oppermann, H., Levinson, A. D. & Varmus, H. E. Virology 108, 47–70 (1980).

    Article  Google Scholar 

  16. Erikson, R. L., Collett, M. S., Erikson, E., Purchio, A. F. & Brugge, J. S. Cold Spring Harb. Symp. quant. Biol. 44, 907–917 (1979).

    Article  Google Scholar 

  17. Levinson, A. D., Oppermann, H., Varmus, H. E. & Bishop, J. M. J. biol. Chem. 255, 11973–11980 (1980).

    CAS  PubMed  Google Scholar 

  18. Smart, J. E., Oppermann, H., Czernilofsky, A. P. & Bishop, J. M. Proc. natn. Acad. Sci. U.S.A. (submitted).

  19. Czernilofsky, A. P. et al. Nature 287, 198–203 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Houmard, J. & Drapeau, G. R. Proc. natn. Acad. Sci. U.S.A. 69, 3506–3510 (1972).

    Article  ADS  CAS  Google Scholar 

  21. Smith, A. E., Smith, R. & Paucha, E. Cell 18, 915–924 (1979).

    Article  CAS  PubMed  Google Scholar 

  22. Eckhart, W., Hutchinson, M. A. & Hunter, T. Cell 18, 925–933 (1979).

    Article  CAS  PubMed  Google Scholar 

  23. Krebs, E. G. & Beavo, J. A. A. Rev. Biochem. 487, 923–959 (1979).

    Article  Google Scholar 

  24. Patschinsky, T. & Sefton, B. M. (in preparation).

  25. Neil, J. C., Breitman, M. & Vogt, P. K. Virology 108, 98–110 (1981).

    Article  CAS  PubMed  Google Scholar 

  26. Smart, J. E. & Ito, Y. Cell 15, 1427–1437 (1979).

    Article  Google Scholar 

  27. Smart, J. E., Lewis, J. B., Mathews, M. B., Harter, M. L. & Anderson, C. W. Virology (in the press).

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Southern California School of Medicine, 2011 Zonal Avenue, Los Angeles, California, 90033, USA

    James C. Neil, Jacques Ghysdael & Peter K. Vogt

  2. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724, USA

    John E. Smart

Authors
  1. James C. Neil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jacques Ghysdael
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter K. Vogt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John E. Smart
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Neil, J., Ghysdael, J., Vogt, P. et al. Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses. Nature 291, 675–677 (1981). https://doi.org/10.1038/291675a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/291675a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing