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Identification of mutations affecting replication control of plasmid Clo DF13

Nature volume 290, pages 264–267 (1981)Cite this article

Abstract

The bacteriocinogenic plasmid Clo DF13, originally isolated from Enterobacter cloacae1, is stably maintained in Escherichia coli2 to the extent of about 10 copies per cell3. Its replication4 resembles that of many other small, multicopy plasmids5–8; plasmid-encoded protein is not required9–12 but plasmid-specific genetic information is involved in regulation of replication as both conditional13 and nonconditional3 copy-number mutants of Clo DF13, and transcomplementable14,15 copy-number mutants of plasmid Col E1 have been described. The sequences essential for replication of Col El (refs 16, 17) and Clo DF13 (refs 18, 19) have been identified within a region surrounding the replication origin. Initiation of Col E1 replication is preceded by transcription of the origin region, providing the RNA primer at the origin20. However, transcription in the opposite direction results in a small transcript of ∼100 nucleotides (RNA-100) for both Col E1 (refs 21, 22) and Clo DF13 (ref. 23). Data suggest that Col E1 RNA-100 acts as a negative control element for the initiation of replication20,24. We show here that single base transitions in the RNA-100 cistron of Clo DF13 can result in a nonconditional increase in plasmid copy-number. Also, sequence analysis has revealed that a specific base transition in a DNA region, apparently involved in both termination and initiation of transcription towards the replication origin, results in a thermosensitive plasmid copy-number.

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References

  1. Tieze, G. A., Stouthamer, A. H., Jansz, H. S., Zandberg, J. & van Bruggen, E. F. J. Molec. gen. Genet. 106, 48–65 (1969).

    Article  CAS  PubMed  Google Scholar 

  2. Kool, A. J., Pranger, M. & Nijkamp, H. J. J. Molec. gen. Genet. 115, 314–323 (1972).

    Article  CAS  PubMed  Google Scholar 

  3. Kool, A. J. & Nijkamp, H. J. J. J. Bact. 120, 569–578 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Veltkamp, E. & Stuitje, A. R. Plasmid (in the press).

  5. Staudenbauer, W. L. Curr. Topics Microbiol. Immun. 83, 93–157 (1978).

    CAS  Google Scholar 

  6. Bolivar, F. et al. Proc. natn. Acad. Sci. U.S.A. 74, 5265–5269 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Conrad, S. E., Wold, M. & Campbell, J. Proc. natn. Acad. Sci. U.S.A. 76, 736–740 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Chang, A. C. Y. & Cohen, S. N. J. Bact. 134, 1141–1156 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Kahn, M. & Helinsky, D. R. Proc. natn. Acad. Sci. U.S.A. 75, 2200–2204 (1978).

    Article  ADS  CAS  Google Scholar 

  10. Donoghue, D. J. & Sharp, P. A. J. Bact. 133, 1287–1294 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Tomizawa, J. Nature 257, 253–254 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Staudenbauer, W. L. Molec. gen. Genet. 145, 273–280 (1976).

    Article  CAS  PubMed  Google Scholar 

  13. Andreoli, P. M., Brandsma, R. A., Veltkamp, E. & Nijkamp, H. J. J. J. Bact. 135, 612–621 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Twigg, A. J. & Sherratt, D. Nature 283, 216–218 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Shepard, H. M., Gelfand, D. H. & Polisky, H. Cell 18, 267–275 (1979).

    Article  CAS  PubMed  Google Scholar 

  16. Oka, A. et al. Molec. gen. Genet. 172, 151–159 (1979).

    Article  CAS  PubMed  Google Scholar 

  17. Backman, K., Betlach, M., Boyer, H. W. & Yanowski, S. Cold Spring Harb. Symp. quant. Biol. 43, 69–76 (1979).

    Article  CAS  PubMed  Google Scholar 

  18. Stuitje, A. R., Veltkamp, E., van den Elzen, P. J. M. & Nijkamp, H. J. J. Nucleic Acids Res. 5, 1801–1820 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Stuitje, A. R., Veltkamp, E., Maat, J. & Heijneker, H. L. Nucleic Acids Res. 8, 1459–1473 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Itoh, T. & Tomizawa, J. Proc. natn. Acad. Sci. U.S.A. 77, 2450–2454 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Patient, R. K. Nucleic Acids Res. 6, 2647–2666 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Morita, M. & Oka, A. Eur. J. Biochem. 97, 435–443 (1979).

    Article  CAS  PubMed  Google Scholar 

  23. Van den Elzen, P. J. M., Konings, R. N. H., Veltkamp, E. & Nijkamp, H. J. J. J. Bact. 144, 579–591 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Conrad, S. E. & Campbell, J. L. Cell 18, 61–71 (1979).

    Article  CAS  PubMed  Google Scholar 

  25. Hashimoto-Gotoh, T. & Inselburg, J. J. Bact. 139, 608–619 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Rosenberg, M. & Court, D. A. Rev. Genet. 13, 319–353 (1979).

    Article  CAS  Google Scholar 

  27. Van den Elzen, P. J. M. et al. Nucleic Acids Res. (in the press).

  28. Stuitje, A. R., Veltkamp, E., Weyers, P. J. & Nijkamp, H. J. J. Nucleic Acids Res. 6, 71–80 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Tinoco, I. et al. Nature new Biol. 246, 44, 40–41 (1973).

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1007 MC, Amsterdam, The Netherlands

    Antoine R. Stuitje, Cornelis E. Spelt, Eduard Veltkamp & H. John J. Nijkamp

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  1. Antoine R. Stuitje
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  2. Cornelis E. Spelt
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  3. Eduard Veltkamp
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  4. H. John J. Nijkamp
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Stuitje, A., Spelt, C., Veltkamp, E. et al. Identification of mutations affecting replication control of plasmid Clo DF13. Nature 290, 264–267 (1981). https://doi.org/10.1038/290264a0

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