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Identification of a glycophorin-like molecule at the cell surface of rat thymocytes

Abstract

There are three predominant glycoproteins in the rat thymocyte plasma membrane. Two of these have carbohydrate compositions that are characteristic of structures N-glycosidically linked to protein. The other glycoprotein is very different, having about 20 O-glycosidically linked carbohydrate units per 100 amino acids. It has similarities to the major sialoglycoprotein of human erythrocytes.

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References

  1. Hughes, R. C. Essays Biochem. 11, 1–36. (1975).

    CAS  PubMed  Google Scholar 

  2. Lloyd, C. W. Biol. Rev. 50, 325–350 (1975).

    Article  CAS  Google Scholar 

  3. Gahmberg, C. G. & Andersson, L. C. J. biol. Chem. 252, 5888–5894 (1977).

    CAS  Google Scholar 

  4. Gahmberg, C. G., Häyry, P. & Andersson, L. C. J. Cell Biol. 68, 642–653 (1976).

    Article  CAS  Google Scholar 

  5. Standring, R., McMaster, W. R., Sunderland, C. A. & Williams, A. F. Eur. J. Immun. 8, 832–839 (1978).

    Article  CAS  Google Scholar 

  6. Standring, R. & Williams, A. F. Biochim. biophys. Acta. 508, 85–96 (1978).

    Article  CAS  Google Scholar 

  7. Letarte-Muirhead, M., Barclay, A. N. & Williams, A. F. Biochem. J. 151, 685–697 (1975).

    Article  CAS  Google Scholar 

  8. Williams, A. F., Barclay, A. N., Letarte-Muirhead, M. & Morris, R. J. Cold Spring Harb. Symp. quant. Biol. 41, 51–61 (1976).

    Article  Google Scholar 

  9. Kuchel, P. W., Campbell, D. G., Barclay, A. N. & Williams, A. F. Biochem. J. 169, 411–417 (1978).

    Article  CAS  Google Scholar 

  10. Campbell, D. G., Gagnon, J., Reid, K. B. M. & Williams, A. F. Biochem. J. (in the press).

  11. Campbell, D. G., Williams, A. F., Bayley, P. M. & Reid, K. B. M. Nature 282, 341–342 (1979).

    Article  ADS  CAS  Google Scholar 

  12. Cohen, F. E., Novotny, J., Sternberg, M. J. E., Campbell, D. G. & Williams, A. F. Biochem. J. (in the press).

  13. Sunderland, C. A., McMaster, W. R. & Williams A. F. Eur. J. Immun. 9, 155–159 (1979).

    Article  CAS  Google Scholar 

  14. Trowbridge, I. S. J. exp. Med. 148, 313–323 (1978).

    Article  CAS  Google Scholar 

  15. Omary, M. B., Trowbridge, I. S. & Scheid, M. P. J. Exp. Med. 151, 1311–1316 (1980).

    Article  CAS  Google Scholar 

  16. Carter, P. B. & Sunderland, C. A. Immunogenetics 10, 583–593 (1980).

    Article  CAS  Google Scholar 

  17. Williams, A. F., Galfrè, G. & Milstein, C. Cell 12, 663–673 (1977).

    Article  CAS  Google Scholar 

  18. Barclay, A. N., Letarte-Muirhead, M., Williams, A. F. & Faulkes, R. Nature 263, 563–567 (1976).

    Article  ADS  CAS  Google Scholar 

  19. Tomita, M., Furthmayr, H. & Marchesi, V. T. Biochemistry 17, 4756–4770 (1978).

    Article  CAS  Google Scholar 

  20. Mason, D. W. & Williams, A. F. Biochem. J. 187, 1–20 (1980).

    Article  CAS  Google Scholar 

  21. Kornfeld, R. & Kornfeld, S. A. Rev. Biochem. 45, 217–237 (1976).

    Article  CAS  Google Scholar 

  22. Iyer, R. N. & Carlson, D. M. Archs Biochem. Biophys. 142, 101–105 (1971).

    Article  CAS  Google Scholar 

  23. Winzler, R. J. in Red Cell Membrane, Structure and Function (eds Jamieson, G A & Greenwalt, T. J.) 157–171 (Lippmcott, Philadelphia, 1969).

    Google Scholar 

  24. Shulte, T. H. & Marchesi, V. T. Biochemistry 18, 275–280 (1979).

    Article  Google Scholar 

  25. Watt, S. M., Burgess, A. W. & Metcalf, D. J. cell. Physiol. 100, 1–22 (1979).

    Article  CAS  Google Scholar 

  26. Nordling, S., Andersson, L. C. & Häyry, P. Science 178, 1001–1002 (1972).

    Article  ADS  CAS  Google Scholar 

  27. Julius, M. H., Simpson, E. & Herzenberg, L. A. Eur. J. Immun. 3, 645–649 (1973).

    Article  CAS  Google Scholar 

  28. Howard, J. C. J. exp Med. 135, 185–199 (1972).

    Article  CAS  Google Scholar 

  29. Komatsu, S. K., DeVries, A. L. & Feeney, R. E. J. biol. Chem. 245, 2909–2913 (1970).

    CAS  PubMed  Google Scholar 

  30. Schmid, K., Mao, S. K. Y., Kimura, A., Hayashi, S. & Binette, J. P. J. biol. Chem. 255, 3221–3226 (1980).

    CAS  PubMed  Google Scholar 

  31. Van den Eijnden, D. H. et al. J. biol. Chem. 254, 12153–12159 (1979).

    CAS  PubMed  Google Scholar 

  32. Sherblom, A. P., Buck, R. L. & Carraway, K. L. J. biol. Chem. 255, 783–790 (1980).

    CAS  PubMed  Google Scholar 

  33. Furthmayr, H. Nature 271, 519–524 (1978).

    Article  ADS  CAS  Google Scholar 

  34. Anstee, D. J., Mawby, W. J. & Tanner, M. J. A. Biochem. J. 183, 193–203 (1979).

    Article  CAS  Google Scholar 

  35. Sarris, A. H. & Palade, G. E. J. biol. Chem. 254, 6724–6731 (1979).

    CAS  PubMed  Google Scholar 

  36. Gahmberg, C. G., Myllyla, G., Leikola, J., Pirkola, A. & Nordlinmg, S. J. biol. Chem. 251, 6108–6116 (1976).

    CAS  PubMed  Google Scholar 

  37. Tanner, M. J. A. & Anstee, J. D. Biochem. J. 153, 271–277 (1976).

    Article  CAS  Google Scholar 

  38. Axén, R. & Ernback, S. Eur. J. Biochem. 18, 351–360 (1971).

    Article  Google Scholar 

  39. Porath, J. Meth. Enzym. 34, 13–29 (1974).

    Article  CAS  Google Scholar 

  40. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).

    CAS  Google Scholar 

  41. Hirs, C. H. W. Meth. Enzym. 11, 59–62 (1967).

    Article  CAS  Google Scholar 

  42. Edelhoch, H. Biochemistry 6, 1948–1954 (1967).

    Article  CAS  Google Scholar 

  43. Liu, T.-Y. & Chang, Y. H. J. biol. Chem. 246, 2842–2848 (1971).

    CAS  PubMed  Google Scholar 

  44. Clamp, J. R. Biochem. Soc. Symp. 40, 3–16 (1974).

    Google Scholar 

  45. Hess, H. H. & Rolde, E. J. biol. Chem. 239, 3215–3220 (1964).

    CAS  PubMed  Google Scholar 

  46. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

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Brown, W., Barclay, A., Sunderland, C. et al. Identification of a glycophorin-like molecule at the cell surface of rat thymocytes. Nature 289, 456–460 (1981). https://doi.org/10.1038/289456a0

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