Abstract
We have determined the order of the mouse immunoglobulin γ1 γ2b, γ2a and ɛ genes by molecular cloning of overlapping chromosomal segments. The results clearly demonstrate that the order is 5′–γ1–(21 kilobases)–γ2b–(15 kilobases)–γ2a–(15 kilobases)–ɛ–3′. There seem to be no J regions at the 5′ side of each constant region gene so far obtained except for the μ gene and these constant region genes seem to have repetitive sequences characteristic of switch (S) regions at their 5′ side.
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References
Landucci-Tosi, S., Mage, R. G. & Dubiski, S. J. Immun. 104, 641–647 (1970).
Kindt, T. J., Mandy, W. J. & Todd, C. W. Biochemistry 9, 2028–2032 (1970).
Knight, K. L. & Hanly, W. C. Contemp. Topics molec. Immun. 4, 55–88 (1975).
Nossal, G. J. V., Warner, N. L. & Lewis, H. Cell Immun. 2, 41–53 (1971).
Fu, S. M., Winchester, R. J. & Kunkel, H. G. J. Immun. 114, 250–252 (1975).
Gearhart, P. J., Sigal, N. H. & Klinman, N. R. Proc. natn. Acad. Sci. U.S.A. 72, 1707–1711 (1975).
Coding, J. W. & Layton, J. E. J. exp. Med. 144, 852–857 (1976).
Sledge, C., Fair, D. S., Black, B., Krueger, R. G. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 73, 923–927 (1976).
Bleux, C., Ventura, M. & Liacopoulos, P. Nature 267, 709–711 (1977).
Wang, A.-C., Wang, I. Y. & Fudenberg, H. H. J. biol Chem. 252, 7192–7199 (1977).
Kincade, P. W., Lawton, A. R., Bockman, D. E. & Cooper, M. D. Proc. natn. Acad. Sci. U.S.A. 67, 1918–1925 (1970).
Honjo, T. & Kataoka, T. Proc. natn. Acad. Sci. U.S.A. 75, 2140–2144 (1978).
Rabbitts, T. H., Forster, A., Dunnick, W. & Bentley, D. L. Nature 283, 351–356 (1980).
Coleclough, C., Cooper, D. & Perry, R. P. Proc. natn. Acad. Sci. U.S.A. 77, 1422–1426 (1980).
Cory, S. & Adams, J. M. Cell 19, 37–51 (1980).
Cory, S., Jackson, J. & Adams, M. Nature 285, 450–456 (1980).
Yaoita, Y. & Honjo, T. Biomed. Res. 1, 164–175 (1980).
Yaoita, Y. & Honjo, T. Nature 286, 850–853 (1980).
Southern, E. M. J. molec. Biol 98, 503–517 (1975).
Maki, R., Traunecker, A., Sakano, H., Roeder, W. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 77, 2138–2142 (1980).
Kataoka, T., Kawakami, T., Takahashi, N. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 77, 919–923 (1980).
Takahashi, N., Kataoka, T. & Honjo, T. Gene 11, 117–127 (1980).
Early, P. W., Huang, H., Davis, M. M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).
Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–683 (1980).
Honjo, T. et al. Cold Spring Harb. Symp. quant. Biol. (in the press).
Davis, M. H. et al. Nature 283, 733–739 (1980).
Honjo, T. et al. Cell 18, 559–568 (1979).
Kataoka, T., Yamawaki-Kataoka, Y., Yamagishi, H. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 76, 4240–4244 (1979).
Tucker, P. W., Marcu, K. B., Newell, N., Richard, J. & Blattner, F. R. Science 206, 1301–1306 (1979).
Yamawaki-Kataoka, Y., Kataoka, T., Takahashi, N., Obata, M. & Honjo, T. Nature 283, 786–789 (1980).
Kawakami, T., Takahashi, N. & Honjo, T. Nucleic Acids Res. 8, 3933–3945 (1980).
Liu, C.-P., Tucker, P. W., Mushinski, J. F. & Blattner, F. R. Science 209, 1348–1353 (1980).
Kataoka, T., Miyata, T. & Honjo, T. Cell (in the press).
Nishida, Y. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Yamawaki-Kataoka, Y. et al. Biochemistry 18, 490–494 (1979).
Sikorav, J.-L., Auffray, C. & Rougeon, F. Nucleic Acids Res. 8, 3143–3155 (1980).
Fougereau, M., Bourgois, A., de Preval, C., Rocca-Serra, J. & Schiff, C. Annls Immun. Inst. Pasteur, Paris 127, 607–631 (1976).
Takahashi, N., Shimizu, A. & Honjo, T. (in preparation).
Fritsch, E. F., Lawn, R. M. & Maniatis, T. Cell 19, 959–972 (1980).
Tsujimoto, Y. & Suzuki, Y. Cell 16, 425–436 (1979).
Davis, M. M., Kim, S. K. & Hood, L. E. Science 209, 1360–1365 (1980).
Maniatis, T. et al. Cell 15, 687–701 (1978).
Blattner, F. R. et al. Science 196, 161–169 (1977).
Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).
Maniatis, T., Jeffrey, A. & Kleid, D. K. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).
Tiemeier, D. C., Tilghman, S. M. & Leder, P. Gene 2, 173–191 (1977).
Obata, M. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Kataoka, T. et al. (in preparation).
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Shimizu, A., Takahashi, N., Yamawaki-Kataoka, Y. et al. Ordering of mouse immunoglobulin heavy chain genes by molecular cloning. Nature 289, 149–153 (1981). https://doi.org/10.1038/289149a0
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DOI: https://doi.org/10.1038/289149a0
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