Letter | Published:

Isolation of human haematopoietic progenitor cells using monoclonal antibodies

Naturevolume 287pages332333 (1980) | Download Citation

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Abstract

Studies of the differentiation of human haematopoietic cells have been greatly advanced by the use of in vitro cloning techniques to enumerate the myeloid and erythroid progenitor cells which form colonies in semi-solid medium1–3. The granulocytic/monocytic colony-forming cells (CFU-GM) and erythroid burst-forming cells (BFU-E) are thought to be mononuclear cells, similar in size to medium size lymphocytes, but their ontogenetic relationship to other progenitor cells and the putative pluripotential stem cell remains obscure4,5. This is partly because elucidation of parent-progeny relationships requires the isolation of precursor cells before they acquire mature phenotypic characters. Physical methods have failed to separate CFU-GM cleanly from other cells because of their heterogeneity, and immunologically specific markers for human progenitor cells have not been reported6,7. We report here that a fraction containing all the granulocytic/monocytic and erythroid progenitor cells, as well as cells which may be lymphoid progenitors, can be isolated from bone marrow using a combination of monoclonal antibodies.

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Author information

Affiliations

  1. ICRF Human Tumour Immunology Group, University College Hospital Medical School, University Street, London, WC1E 6JJ, UK

    • P. C. L. Beverley
  2. Department of Clinical Haematology, University College Hospital Medical School, University Street, London, WC1E 6JJ, UK

    • D. Linch
  3. Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK

    • D. Delia

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https://doi.org/10.1038/287332a0

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