Abstract
Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 µM. During contractures, we recorded Ca2+ transients rising as high as 10 µM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.
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Marban, E., Rink, T., Tsien, R. et al. Free calcium in heart muscle at rest and during contraction measured with Ca2+-sensitive microelectrodes. Nature 286, 845–850 (1980). https://doi.org/10.1038/286845a0
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DOI: https://doi.org/10.1038/286845a0
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