Letter | Published:

A binding site on mast cells and basophils for the anti-allergic drug cromolyn

Nature volume 286, pages 722723 (14 August 1980) | Download Citation

Subjects

Abstract

Calcium permeability of basophil and mast cell membranes is stimulated on allergen binding to its specific membrane-bound IgE1–3. This entry of Ca2+ ions into the cell triggers the degranulation and secretion process4. Disodium cromoglycate (cromolyn DSCG), the disodium salt of l,3-bis(-2-carboxychromon-5-yloxy)-2-hydroxypropane, inhibits the degranulation and release of anaphylactic mediators, and has found wide application in the treatment of allergic bronchial asthma5–7. Accumulated evidence indicates that this inhibition takes place by blocking the calcium uptake8,9. To localize its site of action, the drug has been covalently conjugated to fluorescent poly-acrylamide and polyglutaraldehyde beads (0.7 and 0.2 µm in diameter, respectively)10. We show here that these drug–bead conjugates (DBC) do prevent the drug penetrating into the cell without reducing its ability to inhibit histamine release (Table 1). Furthermore, we show a specific Ca2+-dependent binding of the DBC to the membranes of rat peritoneal mast cells (RPMC) and basophils.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , & J. Physiol., Lond. 271, 193–214 (1977).

  2. 2.

    & J. Physiol., Lond. 239, 381–391 (1974).

  3. 3.

    & Nature 236, 350–351 (1972).

  4. 4.

    , & Nature 245, 249–251 (1973).

  5. 5.

    Nature 216, 1328–1329 (1967).

  6. 6.

    , & Pharmac. exp. Ther. 184, 41–46 (1973).

  7. 7.

    & Clin. exp. Immun. 13, 537–544 (1973).

  8. 8.

    & Br. med. J. 1, 820–821 (1976).

  9. 9.

    & Chem. Pharmac. 25, 505–510 (1976).

  10. 10.

    , & Immun. Meth. 28, 341–353 (1979).

  11. 11.

    Int. Archs Allergy appl. Immun. 60, 356–358 (1979).

  12. 12.

    , & FEBS Lett. 111, 194–196 (1980).

  13. 13.

    , , & Rev. scient. Instrum. 43, 404–409 (1972).

  14. 14.

    & J. Physiol, Lond. 224, 753–769 (1972).

  15. 15.

    , & J. Pharm. exp. Ther. 127, 182–186 (1959).

  16. 16.

    , , & Proc. natn. Acad. Sci. U.S.A. 75, 2659–2663 (1978).

Download references

Author information

Affiliations

  1. Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

    • Nachman Mazurek
    • , Gilad Berger
    •  & Israel Pecht

Authors

  1. Search for Nachman Mazurek in:

  2. Search for Gilad Berger in:

  3. Search for Israel Pecht in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/286722a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.