Letter | Published:

Formation of helical filaments by copolymerization of two types of ‘straight’ flagellins

Nature volume 286, pages 628630 (07 August 1980) | Download Citation

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Abstract

Bacterial flagella undergo transition between several discrete types of left-handed and right-handed helical structures when exposed to acidic1 or alkaline pH2, or to mechanical force3. Calladine4 and ourselves5 have presented models to explain such polymorphism, assuming that protein subunits (flagellin) in a flagellum can be transformed into two conformations (L- and R-states) depending on the species of flagellin and on the environmental conditions. An obvious prediction from these ‘two-state’ models is that there should be two types of straight flagella (L- and R-types) that are made up exclusively of flagel-lins in either the L-state or the R-state. We have shown that straight flagella from two species of mutants, Salmonella SJ814 (ref. 6) and Escherichla coli hag 177 (ref. 7), are closely similar to the predicted R- and L-types, respectively5. Recently we have isolated 10 strains of straight-flagellar mutants of Salmonella. We show here that their flagella can also be classified into the L-and the R-types, and that copolymerization of flagellins from two heterologous types (L and R) makes discrete types of helical filaments, whereas that of homologous pairs of flagellins (L and L, or R and R) makes only straight filaments.

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Author information

Affiliations

  1. Institute of Molecular Biology, School of Science, Nagoya University, Nagoya 464, Japan

    • Ritsu Kamiya
    •  & Sho Asakura
  2. Department of Biology, School of Education, Waseda University, Tokyo 160, Japan

    • Shigeru Yamaguchi

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DOI

https://doi.org/10.1038/286628a0

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