Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus

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Abstract

Pyrimidine dimer formation in response to UV radiation is governed by the thymine content of the potential dimer and the two flanking nucleotides. An enzymatic activity can be purified from Micrococcus luteus that cleaves the N-glycosyl bond between the 5′ pyrimidine of a dimer and the corresponding sugar without rupture of a phosphodiester bond. We propose that strand scission at a dimer site by the M. luteus enzyme requires two activities, a pyrimidine dimer DNA-glycosylase and an apyrimidinic/apurinic endonuclease.

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References

  1. 1

    Hanawalt, P. C. Genetics 79, 179–197 (1975).

  2. 2

    Grossman, L., Braun, A., Feldberg, R. & Mahler, I. A. Rev. Biochem. 44, 19–43 (1975).

  3. 3

    Hamilton, L. D. G., Mahler, I. & Grossman, L. Biochemistry 13, 1886–1896 (1974).

  4. 4

    Braun, A. G., Radman, M. & Grossman, L. Biochemistry 15, 4006–4120 (1976).

  5. 5

    Yasuda, S. & Sekiguchi, M. Proc. natn. Acad. Sci. U.S.A. 67, 1839–1845 (1970).

  6. 6

    Friedberg, E. & King, J. J. Bact. 106, 500–507 (1971).

  7. 7

    Riazuddin, S. & Grossman, L. J. biol. Chem. 252, 6280–6286 (1977).

  8. 8

    Deering, R. A. & Hense, D. S. J. Bact. 121, 1211–1213 (1975).

  9. 9

    Van Lancker, J. A. & Tomura, T. Biochim. biophys. Acta 353, 99–114 (1974).

  10. 10

    Waldstein, E. A., Peller, S. & Setlow, R. B. Proc. natn. Acad. Sci. U.S.A. 76, 3746–3751 (1979).

  11. 11

    Kushner, S. R., Kaplan, J. C., Ono, H. L. & Grossman, L. Biochemistry 10, 3325–3334 (1971).

  12. 12

    Brunk, C. F. Nature new Biol. 241, 74–76 (1973).

  13. 13

    Birnboim, H. C. Photochem. Photobiol. 22, 71–73 (1975).

  14. 14

    Setlow, R. B. & Carrier, W. L. J. molec. Biol. 17, 237–254 (1966).

  15. 15

    DeBoer, G., Pearson, M. L. & Johns, H. E. J. molec. Biol. 27, 131–144 (1967).

  16. 16

    Haseltine, W. A., Lindan, C., D'Andrea, A. & Johnsrud, L. Meth. Enzym. 65, 235–248 (1980).

  17. 17

    Johnsrud, L. Proc. natn. Acad. Sci. U.S.A. 75, 5314–5318 (1978).

  18. 18

    Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

  19. 19

    Riazuddin, S. & Grossman, L. J. biol. Chem. 252, 6287–6293 (1977).

  20. 20

    Wang, S. Y. in Photochemistry and Photobiology of Nucleic Acids, Pyrimidine Biomolecular Products, 296–356 (Academic, New York, 1976).

  21. 21

    D'Andrea, A. D. & Haseltine, W. A. Proc. natn. Acad. Sci. U.S.A. 75, 3608–3612 (1978).

  22. 22

    Carrier, W. L. & Setlow, R. B. J. Bact. 102, 178–186 (1970).

  23. 23

    Shaper, N. L. & Grossman, L. Meth. Enzym. 65, 216–224 (1980).

  24. 24

    Herriott, R. M. Analyt. Biochem. 95, 77–81 (1979).

  25. 25

    Rahn, R. O., Setlow, J. K. & Landry, L. C. Photochem. Photobiol. 18, 39–41 (1973).

  26. 26

    Lamola, A. A. Photochem. Photobiol. 9, 291 (1969).

  27. 27

    Kotchetkov, N. K. & Budovskii, E. I. Organic Chemistry of Nucleic Acids, 542–612 (Plenum, New York, 1972).

  28. 28

    Kelly, R. B., Cozzarelli, L. R., Deutscher, M. P., Lehman, I. R. & Kornberg, A. J. biol. Chem. 245, 39–45 (1970).

  29. 29

    Kaplan, J. C., Kushner, S. R. & Grossman, L. Biochemistry 10, 3315–3324 (1971).

  30. 30

    Chase, J. & Richardson, C. C., J. biol. Chem. 249, 4553–4561 (1974).

  31. 31

    Grossman, L., Riazzudin, S., Haseltine, W. A. & Lindan, C. P. Cold Spring Harb. Symp. quant. Biol. 43, 947–955 (1978).

  32. 32

    Birkner, K. L. & Folk, W. R. J. biol Chem. 252, 3176–3184 (1977).

  33. 33

    Jacobsen, H., Klenow, H. & Overgaard-Hansen, F. Eur. J. Biochem. 45, 623–627 (1974).

  34. 34

    Lindahl, T. & Anderson, A. Biochemistry 11, 3618–3622 (1972).

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