Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus

Nature volume 285, pages 634–641 (1980)Cite this article

Abstract

Pyrimidine dimer formation in response to UV radiation is governed by the thymine content of the potential dimer and the two flanking nucleotides. An enzymatic activity can be purified from Micrococcus luteus that cleaves the N-glycosyl bond between the 5′ pyrimidine of a dimer and the corresponding sugar without rupture of a phosphodiester bond. We propose that strand scission at a dimer site by the M. luteus enzyme requires two activities, a pyrimidine dimer DNA-glycosylase and an apyrimidinic/apurinic endonuclease.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Hanawalt, P. C. Genetics 79, 179–197 (1975).

    PubMed  Google Scholar 

  2. Grossman, L., Braun, A., Feldberg, R. & Mahler, I. A. Rev. Biochem. 44, 19–43 (1975).

    Article  CAS  Google Scholar 

  3. Hamilton, L. D. G., Mahler, I. & Grossman, L. Biochemistry 13, 1886–1896 (1974).

    Article  CAS  Google Scholar 

  4. Braun, A. G., Radman, M. & Grossman, L. Biochemistry 15, 4006–4120 (1976).

    Article  Google Scholar 

  5. Yasuda, S. & Sekiguchi, M. Proc. natn. Acad. Sci. U.S.A. 67, 1839–1845 (1970).

    Article  CAS  ADS  Google Scholar 

  6. Friedberg, E. & King, J. J. Bact. 106, 500–507 (1971).

    CAS  PubMed  Google Scholar 

  7. Riazuddin, S. & Grossman, L. J. biol. Chem. 252, 6280–6286 (1977).

    CAS  PubMed  Google Scholar 

  8. Deering, R. A. & Hense, D. S. J. Bact. 121, 1211–1213 (1975).

    CAS  PubMed  Google Scholar 

  9. Van Lancker, J. A. & Tomura, T. Biochim. biophys. Acta 353, 99–114 (1974).

    Article  CAS  Google Scholar 

  10. Waldstein, E. A., Peller, S. & Setlow, R. B. Proc. natn. Acad. Sci. U.S.A. 76, 3746–3751 (1979).

    Article  CAS  ADS  Google Scholar 

  11. Kushner, S. R., Kaplan, J. C., Ono, H. L. & Grossman, L. Biochemistry 10, 3325–3334 (1971).

    Article  CAS  Google Scholar 

  12. Brunk, C. F. Nature new Biol. 241, 74–76 (1973).

    Article  CAS  ADS  Google Scholar 

  13. Birnboim, H. C. Photochem. Photobiol. 22, 71–73 (1975).

    Article  CAS  Google Scholar 

  14. Setlow, R. B. & Carrier, W. L. J. molec. Biol. 17, 237–254 (1966).

    Article  CAS  Google Scholar 

  15. DeBoer, G., Pearson, M. L. & Johns, H. E. J. molec. Biol. 27, 131–144 (1967).

    Article  CAS  Google Scholar 

  16. Haseltine, W. A., Lindan, C., D'Andrea, A. & Johnsrud, L. Meth. Enzym. 65, 235–248 (1980).

    Article  CAS  Google Scholar 

  17. Johnsrud, L. Proc. natn. Acad. Sci. U.S.A. 75, 5314–5318 (1978).

    Article  CAS  ADS  Google Scholar 

  18. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  CAS  ADS  Google Scholar 

  19. Riazuddin, S. & Grossman, L. J. biol. Chem. 252, 6287–6293 (1977).

    CAS  PubMed  Google Scholar 

  20. Wang, S. Y. in Photochemistry and Photobiology of Nucleic Acids, Pyrimidine Biomolecular Products, 296–356 (Academic, New York, 1976).

    Google Scholar 

  21. D'Andrea, A. D. & Haseltine, W. A. Proc. natn. Acad. Sci. U.S.A. 75, 3608–3612 (1978).

    Article  CAS  ADS  Google Scholar 

  22. Carrier, W. L. & Setlow, R. B. J. Bact. 102, 178–186 (1970).

    CAS  PubMed  Google Scholar 

  23. Shaper, N. L. & Grossman, L. Meth. Enzym. 65, 216–224 (1980).

    Article  CAS  Google Scholar 

  24. Herriott, R. M. Analyt. Biochem. 95, 77–81 (1979).

    Article  CAS  Google Scholar 

  25. Rahn, R. O., Setlow, J. K. & Landry, L. C. Photochem. Photobiol. 18, 39–41 (1973).

    Article  CAS  Google Scholar 

  26. Lamola, A. A. Photochem. Photobiol. 9, 291 (1969).

    Article  CAS  Google Scholar 

  27. Kotchetkov, N. K. & Budovskii, E. I. Organic Chemistry of Nucleic Acids, 542–612 (Plenum, New York, 1972).

    Google Scholar 

  28. Kelly, R. B., Cozzarelli, L. R., Deutscher, M. P., Lehman, I. R. & Kornberg, A. J. biol. Chem. 245, 39–45 (1970).

    CAS  PubMed  Google Scholar 

  29. Kaplan, J. C., Kushner, S. R. & Grossman, L. Biochemistry 10, 3315–3324 (1971).

    Article  CAS  Google Scholar 

  30. Chase, J. & Richardson, C. C., J. biol. Chem. 249, 4553–4561 (1974).

    CAS  PubMed  Google Scholar 

  31. Grossman, L., Riazzudin, S., Haseltine, W. A. & Lindan, C. P. Cold Spring Harb. Symp. quant. Biol. 43, 947–955 (1978).

    Article  Google Scholar 

  32. Birkner, K. L. & Folk, W. R. J. biol Chem. 252, 3176–3184 (1977).

    Google Scholar 

  33. Jacobsen, H., Klenow, H. & Overgaard-Hansen, F. Eur. J. Biochem. 45, 623–627 (1974).

    Article  CAS  Google Scholar 

  34. Lindahl, T. & Anderson, A. Biochemistry 11, 3618–3622 (1972).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sidney Farber Cancer Institute, Harvard University, Boston, Massachusetts, 21205

    William A. Haseltine, Lynn K. Gordon & Christina P. Lindan

  2. Biochemistry Department, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland, 21205

    Robert H. Grafstrom, Nancy L. Shaper & Lawrence Grossman

Authors
  1. William A. Haseltine
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lynn K. Gordon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christina P. Lindan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert H. Grafstrom
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nancy L. Shaper
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lawrence Grossman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haseltine, W., Gordon, L., Lindan, C. et al. Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus. Nature 285, 634–641 (1980). https://doi.org/10.1038/285634a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/285634a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing