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Expression of human α-globin genes in hybrid mouse erythroleukaemia cells depends on differentiated state of human donor cell

Nature volume 285, pages 36–38 (1980)Cite this article

Abstract

We have developed a system which can be used to study the mechanisms that may govern the expression of human α-globin genes in human erythroid and non-erythroid haematopoietic cells1,2. Human chromosome 16, which has been shown to bear the human α-globin genes3, is introduced by cell fusion into mouse erythroleukaemia (MEL) cells to generate continuously proliferating cell lines that retain permanently the human α-globin genes. We have shown that hybrid diploid MEL cells with human α-globin genes from erythroid donor cells express these genes fully through globin chain synthesis, while hybrid diploid MEL cells containing human α-globin genes from non-erythroid human haematopoietic donor cells contain very low levels of human α-globin mRNA and no detectable human α-globin chains. The levels of human α-globin mRNA in these hybrid cells were found to depend on factors present in the MEL recipient cell as well as on the differentiated state of the human donor cell, suggesting that this system may be suitable for characterisation of mechanisms governing haematopoietic differentiation in man.

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Authors and Affiliations

  1. Experimental Hematology Section, Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205

    A. Deisseroth, U. Bode, J. Fontana & D. Hendrick

Authors
  1. A. Deisseroth
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  2. U. Bode
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  3. J. Fontana
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  4. D. Hendrick
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Deisseroth, A., Bode, U., Fontana, J. et al. Expression of human α-globin genes in hybrid mouse erythroleukaemia cells depends on differentiated state of human donor cell. Nature 285, 36–38 (1980). https://doi.org/10.1038/285036a0

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