Abstract
The mechanism of insulin action is still unknown1. One approach to this problem is to apply substances which mimic the action of the hormone to target cells. Ouabain and deprivation of extracellular K+ (refs 2,3), which inhibit the active transport of Na+ and K+ ions, are both known to activate glucose transport and oxidation in isolated adipocytes. Vanadate (V) ions have recently been shown to act as very efficient inhibitors of the sodium pump or (Na+ + K+)ATPase in in vitro preparations4. They have a natriuretic and diuretic effect in rats5 and a positive inotropic effect on cat heart muscle6. Many tissues contain vanadium at a concentration of about 0.1–1.0 μM (ref. 7) and so endogenous vanadate could be a physiological regulator of the sodium pump. But this is still open to debate, because the bulk of the vanadium is probably in the vanadyl (IV) form8,9 and VO2+ ions bind tightly to proteins8,10. VO2+ is a relatively ineffective inhibitor of (Na+ + K+) ATPase in vitro8,11. We report here that externally applied vanadate ions at low concentrations mimic fully the effect of insulin on glucose oxidation in rat adipocytes. However, this simulation seems to be due mainly to the effects of vandyl (IV) ions, probably produced within the cells, and not primarily to inhibition of the sodium pump. Also, externally applied vanadyl (IV) ions stimulate glucose oxidation substantially. Vanadyl ions are known to be powerful inhibitors of alkaline phosphatase12 and we therefore consider the possibility that they inhibit a cellular phosphatase activity. An early event in insulin action may involve alteration of the degree of phosphorylation of protein(s) involved in regulation of sugar transport.
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Shechter, Y., Karlish, S. Insulin-like stimulation of glucose oxidation in rat adipocytes by vanadyl (IV) ions. Nature 284, 556–558 (1980). https://doi.org/10.1038/284556a0
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DOI: https://doi.org/10.1038/284556a0
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