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Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

Abstract

Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.

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References

  1. 1

    Isaacs, A. & Lindenmann, J. Proc. R. Soc. B147, 258–267 (1957).

  2. 2

    Stewart, W. E. II The Interferon System (Springer, Berlin, 1979).

  3. 3

    Lebleu, B., Sen, G. C., Shaila, S., Cabrer, B. & Lengyel, P. Proc. natn. Acad. Sci. U.S.A. 73, 3107–3111 (1976).

  4. 4

    Hovanessian, A. G., Brown, R. E. & Kerr, I. M. Nature 268, 537–540 (1977).

  5. 5

    Hovanessian, A. G. & Kerr, I. M. Eur. J. Biochem. 93, 515–526 (1979).

  6. 6

    Schmidt, A. et al. Proc. natn. Acad. Sci. U.S.A. 76, 4788–4792 (1979).

  7. 7

    Johnson, H. M. Texas Rep. Biol. Med. 35, 357–369 (1978).

  8. 8

    De Maeyer, E. & Maeyer-Guignard, J. Texas Rep. Biol. Med. 35, 370–374 (1978).

  9. 9

    Herberman, R. R., Ortaldo, J. R. & Bonnard, G. D. Nature 277, 221–223 (1979).

  10. 10

    Cavalieri, R. L., Havell, E. A., Vilcek, J. & Pestka, S. Proc. natn. Acad. Sci. U.S.A. 74, 3287–3291 (1977).

  11. 11

    Rubinstein, M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 640–644 (1979).

  12. 12

    Zoon, K. C., Smith, M. E., Bridgen, P. J., zur Nedden, D. & Anfinsen, C. B. Proc. natn. Acad. Sci. U.S.A. 76, 5601–5605 (1979).

  13. 13

    Bridgen, P. J. et al. J. biol. Chem. 252, 6585–6587 (1977).

  14. 14

    Stewart, W. E. II, Wiranowska-Stewart, M., Koistinen, V. & Cantell, K. Virology 97, 473–476 (1979).

  15. 15

    Bose, S., Gurari-Rotman, D., Ruegg, V. T., Corley, L. & Anfinsen, C. B. J. biol. Chem. 251, 1659–1662 (1976).

  16. 16

    Stewart, W. E. II, Lin, L. S., Wiranowska-Stewart, M. & Cantell, K. Proc. natn. Acad. Sci U.S.A. 74, 4200–4204 (1977).

  17. 17

    Villa-Komaroff, L. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3727–3731 (1978).

  18. 18

    Chang, A. C. Y. et al. Nature 275, 617–624 (1978).

  19. 19

    Harpold, M. M., Dobner, P. R., Evans, R. M. & Bancroft, F. C. Nucleic Acids Res. 5, 2039–2053 (1978).

  20. 20

    Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 70, 2330–2334 (1975).

  21. 21

    Cantell, K., Hirvonen, S., Mogensen, K. E. & Pyhälä, L. In Vitro Monogr. 3, 35–38 (1974).

  22. 22

    Hoeijmakers, J. H. J., Borst, P., van den Burg, J., Weissmann, C. & Cross, G. A. M. Gene 8, 391–417 (1980).

  23. 23

    Curtiss, R. III et al. Miami Winter Symp. 13, 99–114 (1977).

  24. 24

    Hanahan, D. & Meselson, M. Gene (in the press).

  25. 25

    Wilkie, N. M. et al. Nucleic Acids Res. 7, 859–877 (1979).

  26. 26

    Mantei, N., Boll, W. & Weissmann, C. Nature 281, 40–46 (1979).

  27. 27

    Gurdon, J. B., Lingrel, J. B. & Marbaix, G. J. molec. Biol. 80, 539–551 (1975).

  28. 28

    Barth, L. G. & Barth, L. J. J. Embryol. exp. Morph. 7, 210–222 (1959).

  29. 29

    Colman, A. & Morser, J. Cell 17, 517–526 (1979).

  30. 30

    Stewart, W. E. II & Sulkin, S. E. Proc. Soc. exp. Biol. Med. 123, 650–653 (1966).

  31. 31

    Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

  32. 32

    Strander, H. & Cantell, K. Ann. Med. exp. Fenn. 44, 265–293 (1966).

  33. 33

    Mogensen, K. E., Pyhälä, L. & Cantell, K. Acta path. microbiol. scand. 83B, 443–450 (1975).

  34. 34

    Cantell, K. & Hirvonen, S. J. gen. Virol. 39, 541–545 (1978).

  35. 35

    Kimchi, A. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3208–3212 (1979).

  36. 36

    Stark, G. R. & Williams, J. G. Nucleic Acids Res. 6, 195–203 (1979).

  37. 37

    Laemmli, U. K. Nature 227, 680–685 (1970).

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