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Contribution of immunoglobulin heavy-chain variable-region genes to antibody diversity

Nature volume 284, pages 238–243 (1980)Cite this article

Abstract

A mouse cloned cDNA probe containing a variable (V) region belonging to the VHIII subgroup has been used in filter hybridisations to estimate the number of heavy-chain V-genes in this subgroup of mouse and human DNA. There seem to be about 10 and 20 VH-genes hybridising to this probe in mouse and human DNA, respectively. Studies of cross-hybridisation of the related VK-genes from MOPC21 and MPC11 myelomas indicate that the experiments detect all members of the VHIII subgroup.

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Author notes

  1. G. Matthyssens

    Present address: Free University Brussels, Institute of Molecular Biology, 65 Paardenstraat, B-1640, St.-Genesius-Rode, Belgium

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    T. H. Rabbitts, G. Matthyssens & P. H. Hamlyn

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  1. T. H. Rabbitts
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  2. G. Matthyssens
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  3. P. H. Hamlyn
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Rabbitts, T., Matthyssens, G. & Hamlyn, P. Contribution of immunoglobulin heavy-chain variable-region genes to antibody diversity. Nature 284, 238–243 (1980). https://doi.org/10.1038/284238a0

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