Article | Published:

Contribution of immunoglobulin heavy-chain variable-region genes to antibody diversity

Abstract

A mouse cloned cDNA probe containing a variable (V) region belonging to the VHIII subgroup has been used in filter hybridisations to estimate the number of heavy-chain V-genes in this subgroup of mouse and human DNA. There seem to be about 10 and 20 VH-genes hybridising to this probe in mouse and human DNA, respectively. Studies of cross-hybridisation of the related VK-genes from MOPC21 and MPC11 myelomas indicate that the experiments detect all members of the VHIII subgroup.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Kabat, E. A., Wu, T. T. & Bilofsky, H. in Sequences of Ig Chains (NIH Publications, 1979).

  2. 2

    Givol, D. Int. Rev. Biochem. IIb, 70–131 (1979).

  3. 3

    Hozumi, N. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 73, 3628–3632 (1976).

  4. 4

    Rabbitts, T. H. & Forster, A. Cell 13, 319–327 (1978).

  5. 5

    Seidman, J. G. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3881–3885 (1978).

  6. 6

    Brack, C., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).

  7. 7

    Sakano, H., Hüppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

  8. 8

    Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

  9. 9

    Seidman, J. G., Max, E. E. & Leder, P. Nature 280, 370–375 (1979).

  10. 10

    Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).

  11. 11

    Seidman, J. G. & Leder, P. Nature 276, 790–795 (1978).

  12. 12

    Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

  13. 13

    Svasti, J. & Milstein, C. Biochem. J. 128, 427–444 (1972).

  14. 14

    Smith, G. P. Biochem. J. 171, 337–347 (1978).

  15. 15

    Hamlyn, P. H., Brownlee, G. G., Cheng, C.-C., Gait, M. J. & Milstein, C. Cell 15, 1067–1075 (1978).

  16. 16

    Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

  17. 17

    Schibler, U., Marcu, K. B. & Perry, R. P. Cell 15, 1495–1509 (1979).

  18. 18

    Rabbitts, T. H., Hamlyn, P. H., Matthyssens, G. & Roe, B. A. in Symp. XIth int. Cong. Biochem. (ed. Kaplan, J. G.) (in the press).

  19. 19

    Rabbitts, T. H., Forster, A., Smith, M. & Gilham, S. Eur. J. Immun. 7, 43–48 (1977).

  20. 20

    Köhler, G. & Milstein, C. Eur. J. Immun. 6, 511–519 (1976).

  21. 21

    Matthyssens, G. & Rabbitts, T. H. Nucleic Acids Res. (in the press).

  22. 22

    Lenhard-Schuller, R., Hohn, B., Brack, C., Hirama, M. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 75, 4709–4713 (1978).

  23. 23

    Kataoka, T., Yamawaki-Kataoka, Y., Yamagishi, H. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 76, 4240–4244 (1979).

  24. 24

    Sakano, H. et al. Nature 277, 627–633 (1979).

  25. 25

    Rao, N., Rudikoff, S., Krutzsch, H. & Potter, M. Proc. natn. Acad. Sci. U.S.A. 76, 2890–2894 (1979).

  26. 26

    Robbiths, T. H., Forster, A., Dunnick, W. & Bentley, O. L. Nature 283, 351–356 (1980).

  27. 27

    Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).

  28. 28

    Denhardt, D. T. Biochem. biophys. Res. Commun. 23, 641–646 (1966).

  29. 29

    Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

  30. 30

    Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1977).

  31. 31

    Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

  32. 32

    Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.