There is now ample evidence for the ubiquity of genetic variation at allozyme loci in many species1,2, but the extent to which this variation is actively maintained by natural selection remains unresolved. Although various approaches have suggested the importance of selection at some allozyme loci in laboratory populations3–6, to be fully convincing, evidence for natural selection must finally come from studies on natural populations. Inevitably, this will entail an understanding of and the potential to manipulate both the ecology and genetics of the population. Because sufficient is known of the ecology, breeding site, nutritional requirements and field behaviour of at least some species of the cactiphilic Drosophila, we7–9 and others10 have chosen them for experimental evaluation of the forces operating to maintain genetic variation. If genetic variation at some locus is being actively maintained by natural selection, the action of this selection should be detectable following artificial change in gene frequencies produced either by adding certain genotypes to the population, or by removing them from it. Jones and Parkin11 have reported unsuccessful attempts to detect selection by such perturbation experiments in Cepaea and Drosophila pseudo-obscura, while Halkka et al.12 inferred strong natural selection at a locus controlling colour polymorphism from exchange of individuals of Philaenus spumarius between two island populations. We report here results for the simultaneous perturbation of gene frequencies at three allozyme loci in Drosophila buzzatii. The changes in gene frequency following this perturbation provide strong evidence for selection, and for differential selection among the three loci.
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Brown, A. H. D. Theor. Popul Biol. 15, 1–42 (1979).
Nevo, E. Theor. Popul Biol 13, 121–177 (1978).
Bijlsma, R. & Van Delden, W. Genet. Res. 30, 221–236 (1977).
Cavener, D. R. & Clegg, M. T. Genetics 90, 629–644 (1978).
Morgan, P. Nature 263, 765–766 (1976).
Oakeshott, J. G. Genet. Res. 26, 265–274 (1976).
Barker, J. S. F. & Mulley, J. C. Evolution 30, 213–233 (1976).
Barker, J. S. F. Lecture Notes Biomath. 19, 403–430 (1977).
Mulley, J. C., James, J. W. & Barker, J. S. F. Biochem. Genet. 17, 105–126 (1979).
Heed, W. B. in Ecological Genetics: The Interface (ed. Brussard, P. F.) 109–126 (Springer, Heidelberg, 1979).
Jones, J. S. & Parkin, D. T. Lecture Notes Biomath. 19, 83–96 (1977).
Halkka, O., Halkka, L. & Raatikainen, M. Hereditas 80, 27–34 (1975).
Wasserman, M. Univ. Texas Publs 6205, 85–117 (1962).
Adams, J. & Ward, R. H. Science 180, 1137–1143 (1973).
About this article
Rapid response to perturbation of chromosome frequencies in natural populations of Drosophila robusta
Molecular Population Genetics of the α-Esterase5 Gene Locus in Original and Colonized Populations of Drosophila buzzatii and Its Sibling Drosophila koepferae
Journal of Molecular Evolution (2007)
The evolutionary history of Drosophila buzzatii. XXXII. Linkage disequilibrium between allozymes and chromosome inversions in two colonizing populations
Perturbation of gene frequencies in a natural population of Drosophila melanogaster: evidence for selection at the Adh locus
Variation in the amount and activity of esterase 6 in a natural population of Drosophila melanogaster