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Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone

Nature volume 281, pages 544–548 (1979)Cite this article

Abstract

DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This ‘hybrid’ gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.

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Authors and Affiliations

  1. Division of Molecular Biology, Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080

    David V. Goeddel, Herbert L. Heyneker, Daniel G. Yansura, Michael J. Ross, Giuseppe Miozzari & Peter H. Seeburg

  2. Division of Organic Chemistry, Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080

    Roberto Crea

  3. Division of Biology, City of Hope National Medical Center, Duarte, California, 91010

    Toyohara Hozumi, Rene Arentzen & Keiichi Itakura

Authors
  1. David V. Goeddel
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  2. Herbert L. Heyneker
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  3. Toyohara Hozumi
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  4. Rene Arentzen
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  5. Keiichi Itakura
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  6. Daniel G. Yansura
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  7. Michael J. Ross
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  8. Giuseppe Miozzari
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  9. Roberto Crea
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  10. Peter H. Seeburg
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Goeddel, D., Heyneker, H., Hozumi, T. et al. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Nature 281, 544–548 (1979). https://doi.org/10.1038/281544a0

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