Abstract
The proliferation of multipotential haematopoietic stem cells (CFU-S) is possible in some long-term bone marrow cultures1–3. Granulocyte and macrophage progenitors (CFU-C)2 and megakaryocyte precursor cells (CFU-M)4 are present in these cultures and undergo full development into mature cells5–7. In contrast, while immature erythroid progenitors (‘early’ BFU-E) are maintained in long-term culture, none of the more differentiated progeny (CFU-E) have been detected, and no morphologically recognisable erythroid cells have been observed8–11. We now describe a modified culture system in which the ‘early’ BFU-E develop into ‘late’ BFU-E in response to added erythropoietin. Further maturation of these cells into CFU-E and non-nucleated erythrocytes can be achieved by mechanical agitation of the long-term cultures or by transferring the cells into dishes which do not allow cell attachment to occur.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Get just this article for as long as you need it
$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Dexter, T. M. & Lajtha, L. G. Br. J. Haemat. 28, 525–530 (1974).
Dexter, T. M. & Testa, N. G. Meth. Cell Biol. 14, 387–405 (1976).
Dexter, T. M., Allen, T. D. & Lajtha, L. G. J. cell. Physiol. 91, 335–344 (1977).
Williams, N. et al. Blood 51, 245–256 (1978).
Allen, T. D. & Dexter, T. M. Differentiation 6, 191–194 (1976).
Williams, N., Jackson, H. & Rabellino, E. J. cell. Physiol. 93, 435–440 (1977).
Allen, T. D. in Stem Cells and Tissue Homeostasis (eds Lord, B. I., Potten, C. S. & Cole, R.) 217–240 (Cambridge University Press, 1978).
Testa, N. G. & Dexter, T. M. Differentiation 9, 193–195 (1977).
Testa, N. G. & Dexter, T. M. in In Vitro Aspects of Erythropoiesis (ed. Murphy, M. J.) 72–74 (Springer, New York, 1978).
Murphy, M. J., Urabe, A. & Sullivan, M. E. in In Vitro Aspects of Erythropoiesis (ed. Murphy, M. J.) 68–71 (Springer, New York, 1978).
Gregory, C. J. & Eaves, A. C. in Differentiation of Normal and Neoplastic Hematopoietic Cells (eds Clarkson, B., Marks, P. A. & Till, J. E.) 179–192 (Cold Spring Harbor Laboratory, New York, 1978).
Greenberger, J. S. Nature 275, 752–755 (1978).
Eliason, J. F., van Zant, G. & Goldwasser, E. Blood 53, 935–945 (1979).
Guilbert, L. J. & Iscove, N. N. Nature 263, 594–595 (1976).
Wagemaker, G. in In Vitro Aspects of Erythropoiesis (ed. Murphy, M. J.) 44–57 (Springer, New York, 1978).
Eliason, J. F. & Goldwasser, E. (in preparation).
Bessis, M. & Breton-Gorius, J. Blood 19, 653–663 (1962).
Ploemacher, R. E. & van Soest, P. L. Cell Tissue Res. 178, 435–461 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eliason, J., Testa, N. & Dexter, T. Erythropoietin-stimulated erythropoiesis in long-term bone marrow culture. Nature 281, 382–384 (1979). https://doi.org/10.1038/281382a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/281382a0
This article is cited by
-
Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo
Nature Biotechnology (2002)
-
Hemopoiesis in long-term stroma-dependent cultures from lymphoid tissue: Production of cells with myeloid/dendritic characteristics
In Vitro Cellular & Developmental Biology - Animal (1998)
-
Long-term survival of murine erythroid progenitors in long-term bone marrow culture and stromal cell culture: differentiation in peritoneal diffusion chamber culture
Blut (1989)
-
Myelo-lymphopoiesis in long-term bone marrow culture
Klinische Wochenschrift (1985)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
