Abstract
Although brome mosaic virus RNA3 has only one translatable cistron, it can bind two 80S ribosomes at initiation. One ribosome binds at the first AUG codon (bases 92–94). The other binds nearer the 5′ end at an entry or holding site. Disome formation is thus unrelated to a silent cistron ∼1,000 bases downstream.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kozak, M. Cell 15, 1109–1123 (1978).
Baralle, F. E. and Brownlee, G. G. Nature 274, 84–87 (1978).
Shatkin, A. J. Cell 9, 645–653 (1976).
Rose, J. K. & Lodish, H. F. Nature 262, 32–37 (1976).
Davies, J. W. & Kaesberg, P. J. Virol. 12, 1434–1441 (1973).
Lane, L. C. & Kaesberg, P. Nature new Biol. 232, 40–43 (1971).
Shih, D. S. & Kaesberg, P. Proc. natn. Acad. Sci. U.S.A. 70, 1799–1803 (1973); J. molec. Biol. 103, 77–88 (1976).
Knowland, J., Hunter, T., Hunt, T. & Zimmern, D. INSERM 47, 211–216 (1975).
Van Vloten-Doting, L. et al., Nucleic Acids and Protein Synthesis in Plants (eds Bogorad, L. & Weil, J. H.) (Plenum, New York, 1977).
Vazquez, D., Battaner, E., Neth, R., Heller, G. & Monro, R. E. Cold Spring Harb. Symp. quant. Biol. 34, 369–375 (1969).
Lodish, H. F. J. biol. Chem. 246, 7131–7138 (1971).
Smith, K. E., Cannon, M. & Cundliffe, E. FEBS Lett. 50, 8–12 (1975).
Dasgupta, R., Shih, D. S., Saris, C. & Kaesberg, P. Nature 256, 624–628 (1975).
Moss, B. Biochem. biophys. Res. Commun. 74, 374–383 (1977).
Reeder, R. H., Sollner-Webb, B. & Wahn, H. L. Proc. natn. Acad. Sci. U.S.A. 74, 5402–5406 (1977).
Muthukrishnan, S., Moss, B., Cooper, J. & Maxwell, E. J. biol. Chem. 253, 1710–1715 (1978).
Chu, L.-Y., Lockard, R. E., RajBhandary, U. L. & Rhoads, R. E. J. biol. Chem. 253, 5228–5231 (1978).
Donis-Keller, H., Maxam, A. & Gilbert, W. Nucleic Acids Res. 4, 2527–2538 (1977).
Simoncsits, A., Brownlee, G. G., Brown, R. S., Rubin, J. R. & Guilley, H. Nature 269, 833–836 (1977).
Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).
Silberklang, M., Gillum, A. M. & RajBhandary, U. L. Nucleic Acids Res. 4, 4091–4108 (1977).
Pilly, D., Niemeyer, A., Schmidt, M. & Bargetzi, J. P. J. biol. Chem. 253, 437–445 (1978).
Krol, A., Machatt, M., Branlant, C. & Ebel, J. Nucleic Acids Res. 5, 4933–4947 (1978).
Hunter, A. R., Jackson, R. J. & Hunt, T. Eur. J. Biochem. 75, 159–170 (1977).
Kozak, M. & Shatkin, A. J. J. biol. Chem. 252, 6895–6908 (1977).
Both, G. W., Furuichi, Y., Muthukrishnan, S. & Shatkin, A. J. J. molec. Biol. 104, 637–658 (1976).
Kozak, M. & Shatkin, A. J. J. biol. Chem. 253, 6568–6577 (1978).
Richards, K., Guilley, H., Jonard, G. & Hirth, L. Eur. J. Biochem. 84, 513–519 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ahlquist, P., Dasgupta, R., Shih, D. et al. Two-step binding of eukaryotic ribosomes to brome mosaic virus RNA3. Nature 281, 277–282 (1979). https://doi.org/10.1038/281277a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/281277a0
This article is cited by
-
Translation in plants-rules and exceptions
Plant Molecular Biology (1996)
-
The potential exploitation of plant viral translational enhancers in biotechnology for increased gene expression
Molecular Biotechnology (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.