Abstract
A three-dimensional image of tubulin protofilaments, reconstructed to 20 Å resolution from electron micrographs of negatively stained zinc-induced sheets, has been used to generate an improved model for microtubule substructure. This model has been used to phase a reconstructed image from X-ray amplitudes, which is compatible with the electron microscope results.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Chasey, D. Expl Cell Res. 74, 140–146 (1972).
Erickson, H. P. J. Cell Biol. 60, 153–167 (1974).
Amos, L. A. & Klug, A. J. Cell Sci. 14, 523–549 (1974).
Crepeau, R. H., McEwen, B., Dykes, G. & Edelstein, S. J. J. molec. Biol. 116, 301–315 (1977).
Cohen, C., Harrison, S. C. & Stephens, R. E. J. molec. Biol. 59, 375–380 (1971).
Cohen, C., DeRosier, D. J., Harrison, S. C., Stephens, R. E. & Thomas, J. Ann. N.Y. Acad. Sci. 253, 53–59 (1975).
Mandelkow, E., Thomas, J. & Cohen, C. Proc. natn. Acad. Sci. U.S.A. 74, 3370–3374 (1977).
Larsson, H., Wallin, M. & Edstrom, A. Expl Cell Res. 100, 104–110 (1976).
Gaskin, F. & Kress, Y. J. biol. Chem. 252, 6918–6924 (1977).
Kirschner, M. W., Williams, R. C., Weingarten, M. & Gerhart, J. C. Proc. natn. Acad. Sci. U.S.A. 71, 1159–1163 (1974).
Baker, T. S. & Amos, L. A. J. molec. Biol. 123, 89–106 (1978).
Crepeau, R. H., McEwen, B. & Edelstein, S. J. Proc. natn. Acad. Sci. U.S.A. 75, 5006–5010 (1978).
Amos, L. A. & Baker, T. S. Int. J. biol. Macromolec. (submitted).
Henderson, R. & Unwin, P. N. T. Nature 257, 28–32 (1975).
Luduena, R. F., Shooter, E. M. & Wilson, L. J. biol. Chem. 252, 7006–7014 (1977).
Tilney, L. G. et al. J. Cell Biol. 59, 267–275 (1973).
Pierson, G. B., Burton, P. R. & Himes, R. H. J. Cell Biol. 76, 223–228 (1978).
Warner, F. D. & Satir, P. J. Cell Sci. 12, 313–326 (1974).
Amos, L. A. J. Cell Biol. 72, 642–654 (1977).
Amos, L. A. in Microtubules (eds Roberts, K. & Hyams, J. S.) (Academic, London, 1979).
Unwin, P. N. T. & Henderson, R. J. molec. Biol. 94, 425–440 (1975).
Unwin, P. N. T. J. molec. Biol. 98, 235–242 (1975).
Holmes, K. C., Stubbs, G. J., Mandelkow, E. & Gallwitz, U. Nature 254, 192–196 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Amos, L., Baker, T. The three-dimensional structure of tubulin protofilaments. Nature 279, 607–612 (1979). https://doi.org/10.1038/279607a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/279607a0
This article is cited by
-
Comparison of microtubules stabilized with the anticancer drugs cevipabulin and paclitaxel
Polymer Journal (2020)
-
Tubulin and FtsZ form a distinct family of GTPases
Nature Structural Biology (1998)
-
Taxol found on tubulin
Nature (1995)
-
Structure of tubulin at 6.5 Å and location of the taxol-binding site
Nature (1995)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.