Identification and change of collagen types in differentiating myoblasts and developing chick muscle

Abstract

COLLAGEN is the major structural protein in the supporting and connective tissues. In addition to its mechanical properties it has been considered to be involved in developmental processes1–3. Previous studies on muscle development have concentrated on the contractile components but more recently there has been a realisation of the significance of connective tissue in this process. For example, in vitro studies have demonstrated the importance of a collagen substrate in promoting myoblast differentiation4, and more recently it was shown that all types of collagen were equally effective5: Mayne et al.6 demonstrated that clones of chick muscle cells were capable of synthesising type I collagen. Ketley et al.5 confirmed the synthesis of type I collagen and also reported that the collagen in intact chick muscle was solely of this type. On the other hand, Duance et al.7 and Bailey and Sims8 investigated the distribution of the genetically different collagen types within bovine muscle connective tissue and showed by biochemical and immunofluorescent techniques a specific distribution of the isomorphic forms of collagen in this tissue. Here we report our studies on the development of chick skeletal muscle which were designed to investigate some pertinent points: (1) the distribution of collagen types in chick muscle; (2) the role of collagen in the development of muscle as a functional tissue; (3) the cellular derivation of these different collagens. Chick muscle was chosen, first, because much is known about its development and differentiation in vitro, and second, because it is possible to manipulate its early development in the embryo in vivo9,10. We have isolated three isomorphic forms of collagen from chick muscle, and using immunofluorescent techniques demonstrate a specific distribution of these collagen types within this tissue. Finally we have used in vitro muscle cell culture to examine how this distribution is achieved by temporal transitions in synthesis. These results suggest that collagen has a subtle role in the development of muscle.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Weiss, P. in Biological Organisation (ed. Waddington, C. H.) (Pergamon, Oxford, 1959).

  2. 2

    Grobstein, C. in Aspects of Synthesis in Order and Growth (ed. Rudnick, D.) (Princeton University Press, 1954).

  3. 3

    Fitton-Jackson, S. in Treatise on Collagen Vol. 28 (ed. Gould, B. S.) (Academic, New York, 1968).

  4. 4

    Hauschka, S. D. & Konigsberg, I. R. Proc. natn. Acad. Sci. U.S.A. 55, 119–126 (1966).

  5. 5

    Ketley, J. N., Orkin, R. W. & Martin, G. R. Expl Cell Res. 99, 261–268 (1976).

  6. 6

    Mayne, R. & Strahs, K. R. J. Cell Biol. 63, 212a (1974).

  7. 7

    Duance, V. C., Restall, D. J., Beard, H., Bourne, F. J. & Bailey, A. J. FEBS Lett. 79, 248–252 (1977).

  8. 8

    Bailey, A. J. & Sims, T. J. J. Sci. Fd Agric. 28, 565–570 (1977).

  9. 9

    Shellswell, G. B. & Wolpert, L. in Vertebrate Limb and Somite Morphogenesis (eds Ede, D., Hinchcliffe, R. & Balls, M.) (Cambridge University Press, 1977).

  10. 10

    Shellswell, G. B. thesis, Univ. London (1977).

  11. 11

    Burgeson, R. E., El Adli, F. A., Kartila, I. I. & Hollister, D. A. Proc. natn. Acad. Sci. U.S.A. 73, 2579–2583 (1976).

  12. 12

    Bentz, H., Bächinger, H. P., Glanville, R. & Kuhn, K. Eur. J. Biochem. 92, 563–568 (1978).

  13. 13

    Rhodes, K. R. & Miller, E. J. Biochemistry 17, 3443–3448 (1978).

  14. 14

    Lipton, B. H. Devl Biol. 61, 153–165 (1977).

  15. 15

    Wolpert, L. Sci. Am. 239, 124–137 (1978).

  16. 16

    Wolpert, L. Curr. Top. dev. Biol. 6, 183–224 (1971).

  17. 17

    Yaffe, D. Curr. Top. dev. Biol. 4, 37–75 (1969).

  18. 18

    Herbert, W. J. in Handbook of Experimental Immunology (ed. Weir, D. M.) (Blackwell, Oxford, 1967).

  19. 19

    Sykes, B., Puddle, B., Francis, M. & Smith, R. Biochem. biophys. Res. Commun. 72, 1472–1480 (1976).

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

BAILEY, A., SHELLSWELL, G. & DUANCE, V. Identification and change of collagen types in differentiating myoblasts and developing chick muscle. Nature 278, 67–69 (1979). https://doi.org/10.1038/278067a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.