Abstract
ORGAN culture of nerve and muscle facilitates investigations which cannot easily be carried out in situ1,2. We have developed a method for maintaining human muscle in organ culture which should allow us to investigate normal as well as various pathological properties of muscle fibres. We report here the use of these preparations to compare junctional and extrajunctional channels, induced by acetylcholine (ACh), in human muscle. In addition we have made a preliminary study of miniature endplate currents, in normal and myasthenia gravis endplates, following organ culture.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Nelson, P. G. Physiol. Rev. 55, 1–61 (1975).
Miledi, R. & Trowell, O. A. Nature 194, 981–982 (1962).
Purves, D. & Sakmann, B. J. Physiol., Lond. 237, 157–182 (1974).
Devreotes, P. N. & Fambrough, D. M. J. Cell Biol. 65, 335–358 (1975).
Cull-Candy, S. G., Miledi, R. & Trautmann, A. Nature 271, 74–75 (1978).
Katz, B. & Miledi, R. J. Physiol., Lond. 170, 379–388 (1964).
Miledi, R. & Zelen´, J. Nature 210, 855–856 (1966).
Katz, B. & Miledi, R. J. Physiol., Lond. 224, 665–700 (1972).
Anderson, C. R. & Stevens, C. F. J. Physiol., Lond. 235, 655–691 (1973).
Colquhoun, D. & Hawkes, A. G. Proc. R. Soc. B 199, 231–262 (1977).
Cull-Candy, S. G., Miledi, R. & Trautmann, A. J. Physiol., Lond. 287 (in the press).
Engel, A. G., Lambert, E. H. & Howard, F. M. Mayo Clin. Proc. 52, 267–280 (1977).
Fambrough, D. M., Drachmann, D. B. & Satyamurti, S. Science 182, 293–295 (1973).
Green, D. P. L., Miledi, R. Perez de la Mora, M. & Vincent, A. Phil. Trans. R. Soc. B270, 551–559 (1975).
Bevan, S., Kullberg, R. W. & Heinemann, S. F. Nature 267, 263–265 (1977).
Heinemann, S., Merlie, J. & Lindstrom, J. Nature 274, 65–68 (1978).
Reiness, C. G., Weinberg, C. B. & Hall, Z. W. Nature 274, 68–70 (1978).
Newsom Davis, J., Pinching, A. J., Vincent, A. & Wilson, S. G. Neurology, 28, 266–272 (1978).
Neher, E. & Sakmann, B. J. Physiol., Lond. 258, 705–729 (1976).
Dreyer, F., Müller, K. D., Peper, K. & Sterz, R. Pflugers Arch. ges. Physiol. 367, 115–122 (1976).
Bevan, S., Kullberg, R. W. & Rice, J. Nature 273, 469–471 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CULL-CANDY, S., MILEDI, R. & UCHITEL, O. Acetylcholine receptors in organ-cultured human muscle fibres. Nature 277, 236–238 (1979). https://doi.org/10.1038/277236a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/277236a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.