Letter | Published:

Primary sensitisation and restimulation of human lymphocytes with soluble antigen in vitro

Naturevolume 277pages223225 (1979) | Download Citation



RESEARCH with human lymphocytes is restricted by ethical constraints on sensitising individuals in vivo before in vitro experiments. In humans specific restimulation of proliferative responses was first shown using allogeneic lymphocytes as antigen and has since been developed into the primed lymphocyte test1. In a model similar to this test, again using cell-bound antigens, Newman et al.2 showed sensitisation and restimulation by hapten-modified autologous cells. With soluble protein antigens and lymphocytes from donors who had been sensitised to these antigens in vivo, Levis and Dattner3 showed specific restimulation of proliferative responses, after the cells had been cultured in the presence of antigen for 10 or more days. We report here that, using a similar culture method, but cells from individuals who have not been sensitised in vivo to the relevant protein antigens, we have been able to obtain in vitro sensitisation and specific restimulation, sensitisation of the lymphocytes being determined by a subsequent specific proliferative response.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Sheehy, M. J., Sondel, P. M., Bach, M. L., Wank, R. & Bach, F. H. Science 188, 1308–1310 (1975).

  2. 2

    Newman, W., Stoner, G. L. & Bloom, B. R. Nature 269, 151–153 (1977).

  3. 3

    Levis, W. R. & Dattner, A. M. Lancet i, 875–877 (1977).

  4. 4

    Thomas, D. W. & Shevach, E. M. J. exp. Med. 144, 1263–1273 (1976).

  5. 5

    Dosch, H. & Gelfand, E. W. J. Immun. 118, 302–308 (1977).

  6. 6

    UytdeHaag, F., Heijnen, C. J. & Ballieux, R. E. Nature 271, 556–557 (1978).

Download references

Author information


  1. Department of Immunohaematology, University Hospital, Leiden, The Netherlands

    • E. J. HENSEN
    •  & B. G. ELFERINK


  1. Search for E. J. HENSEN in:

  2. Search for B. G. ELFERINK in:

About this article

Publication history



Issue Date




By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.