Letter | Published:

Activation of adenylate cyclase by vanadate

Naturevolume 277pages143145 (1979) | Download Citation

Subjects

Abstract

VANADATE has been identified as a potent inhibitor of (Na,K)ATPases1,2. The (Na,K)ATPase of dog kidney is inhibited 50% by 40 nM sodium orthovanadate (Na2VO4) in optimal conditions (28 mM Mg2+)2. As vanadate was originally identified as an inhibitor in muscle-derived ATP preparations2 and in equine and rabbit skeletal muscles, it has been suggested as endogenous regulator of (Na,K)ATPase2–4. We have tried to use vanadate as a tool to inhibit ATPases in adenylate cyclase studies when working at low ATP concentrations. We report here that vanadate causes marked stimulation of adenylate cyclase activity in membranes isolated from rat fat cells and produces maximal activation even in the absence of hormones.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Josephson, L. & Cantley, L. C. Biochemistry 16, 4572–4578 (1977).

  2. 2

    Cantley, L. C. et al. J. biol. Chem. 252, 7421–7423 (1977).

  3. 3

    Beauge, L. A. & Glynn, I. M. Nature 272, 551–552 (1978).

  4. 4

    Cantley, L. C., Resh, M. D. & Guidotti, G. Nature 272, 552–554 (1978).

  5. 5

    McKeel, D. W. & Jarett, L. J. Cell Biol. 44, 417–432 (1970).

  6. 6

    Schwabe, U., Ebert, R. & Schönhöfer, P. S. Naunyn-Schmiedebergs Archs Pharmak. 286, 83–96 (1974).

  7. 7

    Gilman, A. G. Proc. natn. Acad Sci. U.S.A. 67, 305–312 (1970).

  8. 8

    Cassel, D. & Selinger, Z. Proc. natn. Acad Sci. U.S.A. 74, 3307–3311 (1977).

  9. 9

    Levinson, S. L. & Blume, A. J. J. biol. Chem. 252, 3766–3774 (1977).

  10. 10

    Hackbarth, I. et al. Nature 275, 67 (1978).

  11. 11

    Jackson, D. E. J. Pharmac. exp. Ther. 3, 477–514 (1911).

Download references

Author information

Affiliations

  1. Institut für Pharmakologie und Toxikologie, Medizinische Hochschule Hannover, Karl-Wiechert-Allee 9, 3000, Hannover, 61, FRG

    • ULRICH SCHWABE
    • , CHRISTOPH PUCHSTEIN
    • , HELGA HANNEMANN
    •  & EICKE SÖCHTIG

Authors

  1. Search for ULRICH SCHWABE in:

  2. Search for CHRISTOPH PUCHSTEIN in:

  3. Search for HELGA HANNEMANN in:

  4. Search for EICKE SÖCHTIG in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/277143a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.