Abstract
WE have previously reported that pathogenicity of Vibrio cholerae was suppressed by introducing P or V plasmids and that this effect was due to decreased toxin production1. This work has been extended to show that cells harbouring both P and V plasmids become attenuated and do not cause experimental cholera. Such attenuated strains are attractive candidates for live oral vaccines if it can be shown that the immunogenicity of plasmid-harbouring strains is not altered and that they remain stable. We provide here convincing evidence that the cells are not cured of their plasmids in vitro or in vivo and do not lose their immunogenic potential.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Sinha, V. B. & Srivastava, B. S. J. gen. Microbiol. 104, 251–255 (1978).
Bhaskaran, K. & Sinha, V. B. J. gen. Microbiol. 40, 281–284 (1971).
De, S. N. & Chatterjee, D. N. J. path. Bact. 66, 559–562 (1953).
Dutta, N. K. & Habbu, M. K. Br. J. Pharmac. 10, 153–159 (1955).
Parker, C., Gauthier, D., Tate, A. & Romig, W. R. Symposium on Cholera (eds Fukumi, H. & Ohashi, M.) 24–34 (US-Japan Cooperative Medical Science Programme, NIH, Tokyo, 1972).
Hayes, W. The Genetics of Bacteria and Their Viruses (Blackwell, Oxford, 1968).
Bhaskaran, K. Cholera (eds Barua, D. & Burrows, W.) 41–59 (W. B. Saunders, Philadelphia, 1974).
Pittman, M. & Feeley, J. C. Proc. Cholera Res. Symp. (eds Bushnell, O. A. & Brookhyser, C. S.) 163–167 (US Govt Printing Office, Washington, 1965).
Joo, I. Cholera (eds Barua, D. & Burrows, W.) 333–355 (W. B. Saunders, Philadelphia 1974).
Sinha, V. B. & Bhaskaran, K. Bull. Wld Hlth Org. 49, 605–613 (1973).
Finkelstein, R. A. J. Immun. 89, 264–271 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SINHA, V., SRIVASTAVA, B. Plasmid-induced loss of virulence in Vibrio cholerae. Nature 276, 708–709 (1978). https://doi.org/10.1038/276708a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/276708a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.