Abstract
THE cI gene of bacteriophage λ codes for the λ represser. This protein is both a positive and a negative regulator of gene transcription (see ref. 1 for review). These functions are mediated by the interaction of represser with two operators in the phage DNA. Both the nucleotide sequence of the λ operators2–5 and the amino acid sequence of the λ represser have been determined6. The ability of the λ represser to bind operator DNA is greatly reduced by a proteolytic cleavage which occurs, for example, during recA-dependent prophage induction in Escherichia coli7. A single mutation (λcI ind−) prevents this cleavage and inactivation of represser7,8. I present here the nucleotide sequence of the λ cI gene and identify the base change responsible for the ind− phenotype of λ represser.
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SAUER, R. DNA sequence of the bacteriophage λ cI gene. Nature 276, 301–302 (1978). https://doi.org/10.1038/276301a0
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DOI: https://doi.org/10.1038/276301a0
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