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Sequences from the genome of a non-transforming mutant of polyoma virus

Nature volume 276, pages 294–298 (1978)Cite this article

Abstract

POLYOMA, the small DNA animal tumour virus, like the SV40 virus, has attracted interest because its genome is small enough to be analysed at the molecular level1–6, enabling study of the molecular basis of oncogenic transformation. In polyoma virus there are several deletion mutants, designated hr-t (host-range transformation) mutants, which are unable to transform cells (or cause tumours) and which will grow in certain types of cells only, mainly those which have already been transformed. We report here that we have sequenced the region of the DNA which probably determines the non-transforming phenotype of one such hr-t mutant (NG-18)7 and compared it with the sequence of wild-type polyoma virus DNA. These studies show NG-18 to be a frame-shift mutant, and to have lost 187 of the nucleotides present in wild-type DNA, including several nucleotide triplets which code for cysteine.

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References

  1. Soeda, E., Miura, K., Nakaso, A. & Kimura, G. FEBS Lett. 79, 383–389 (1977).

    Article  CAS  Google Scholar 

  2. Soeda, E., Kimura, G. & Miura, K. I. Proc. natn. Acad. Sci. U.S.A. 75, 162–166 (1978).

    Article  ADS  CAS  Google Scholar 

  3. Soeda, E., Arrand, J. A., Smolar, N. & Griffin, B. E. (in preparation).

  4. Fried, M. & Griffin, B. E. Adv. Cancer Res. 24, 67–113 (Academic, New York, 1977).

    Article  CAS  Google Scholar 

  5. Fiers, W. et al. Nature 273, 113–120 (1978).

    Article  ADS  CAS  Google Scholar 

  6. Reddy, V. B. et al. Science 200, 494–502 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Benjamin, T. C. Proc. natn. Acad. Sci. U.S.A. 67, 394–399 (1970).

    Article  ADS  CAS  Google Scholar 

  8. Feunteun, J., Sompayrac, L., Fluck, M. & Benjamin, T. Proc. natn. Acad. Sci. U.S.A. 73, 4169–4173 (1976).

    Article  ADS  CAS  Google Scholar 

  9. Ito, Y., Brocklehurst, J. R., Spurr, N., Griffiths, M. & Fried, M. EMBO-INSERM Workshop on Early Proteins of Oncogenie DNA Viruses, Thiverval-Grignon, France (1977).

  10. Schaffhausen, B. S., Silver, J. E. & Benjamin, T. C. Proc. natn. Acad. Sci. U.S.A. 75, 79–83 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Ito, Y., Spurr, N. & Dulbecco, R. Proc. natn. Acad. Sci. U.S.A. 74, 1259–1263 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Eckhart, W. Virology 77, 589–597 (1977).

    Article  CAS  Google Scholar 

  13. Fluck, M. M., Staneloni, R. J. & Benjamin, T. L. Virology 77, 610–624 (1977).

    Article  CAS  Google Scholar 

  14. Smart, J. E. & Ito, Y. Cell (in the press).

  15. Griffin, B. E., Fried, M. & Cowie, A. Proc. natn. Acad. Sci. U.S.A. 71, 2077–2081 (1974).

    Article  ADS  CAS  Google Scholar 

  16. Ito, Y., Brocklehurst, J. R. & Dulbecco, R. Proc. natn. Acad. Sci. U.S.A. 74, 4666–4670 (1977).

    Article  ADS  CAS  Google Scholar 

  17. Staneloni, R. J., Fluck, M. M. & Benjamin, T. C. Virology 77, 598–609, (1977).

    Article  CAS  Google Scholar 

  18. Volckaert, G. Van de Voorde, A. & Fiers, W. Proc. natn. Acad. Sci. U.S.A. 75, 2160–2164 (1978).

    Article  ADS  CAS  Google Scholar 

  19. Berget, S. M., Moore, C. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1977).

    Article  ADS  CAS  Google Scholar 

  20. Berk, A. J. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 75, 1274–1278 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Goulian, M., Lucas, Z. J. & Kornberg, A. J. biol. Chem. 10, 627–638 (1968).

    Google Scholar 

  22. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  23. Griffin, B. E. J. molec. Biol. 117, 447–471 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–110 (1978).

    Article  CAS  Google Scholar 

  25. Kamen, R., Sedat, J. & Ziff, E. J. Virol. 17, 212–218 (1976).

    CAS  PubMed Central  Google Scholar 

  26. Shenk, T., Carbon, J. & Berg, P. J. Virol. 18, 664–671 (1976).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Crawford, L. V. et al. Proc. natn. Acad. Sci. U.S.A. 75, 117–121 (1978).

    Article  ADS  CAS  Google Scholar 

  28. Bouck, N., Beales, N., Shenk, T., Berg, P. & di Mayorca, G. Proc. natn. Acad. Sci. U.S.A. 75, 2473–2477 (1978).

    Article  ADS  CAS  Google Scholar 

  29. Sleigh, M. J., Topp, W. C., Hanich, R. & Sambrook, J. F. Cell 14, 79–88 (1978).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2, UK

    EIICHI SOEDA & BEVERLY E. GRIFFIN

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  1. EIICHI SOEDA
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  2. BEVERLY E. GRIFFIN
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SOEDA, E., GRIFFIN, B. Sequences from the genome of a non-transforming mutant of polyoma virus. Nature 276, 294–298 (1978). https://doi.org/10.1038/276294a0

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