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Transformation of yeast by a replicating hybrid plasmid

Abstract

Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from E. coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.

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References

  1. 1

    Hinnen, A., Hicks, J. B., Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Guerineau, M., Grandchamp, C., Paoletti, C. & Slonimski, P. P. Biochem. biophys. Res. Commun. 42, 550–557 (1971).

    CAS  Article  Google Scholar 

  3. 3

    Stevens, B. J. & Moustacchi, E. Expl Cell Res. 64, 259–266 (1971).

    CAS  Article  Google Scholar 

  4. 4

    Bak, A. L., Christiansen, C. & Christiansen, G. Biochim. biophys. Acta 269, 527–530 (1972).

    CAS  Article  Google Scholar 

  5. 5

    Clark-Walker, G. D. Eur. J. Biochem. 32, 263–267 (1973).

    CAS  Article  Google Scholar 

  6. 5a

    Clark-Walker, G. D. Proc. natn. Acad. Sci. U.S.A. 69, 388–392 (1972).

    ADS  CAS  Article  Google Scholar 

  7. 6

    Beggs, J. D., Guerineau, M. & Atkins, J. F. Molec. gen. Genet. 148, 287–294 (1976).

    CAS  Article  Google Scholar 

  8. 7

    Petes, T. D. & Williamson, D. H. Cell 4, 249–253 (1975).

    CAS  Article  Google Scholar 

  9. 8

    Livingston, D. M. & Kupfer, D. M. J. molec. Biol. 116, 249–260 (1977).

    CAS  Article  Google Scholar 

  10. 9

    Clark-Walker, G. D. & Miklos, G. L. G. Eur. J. Biochem. 41, 359–365 (1974).

    CAS  Article  Google Scholar 

  11. 10

    Hollenberg, C. P., Degelmann, A., Kustermann-Kuhn, B. & Royer, H. D. Proc. natn. Acad. Sci. U.S.A. 73, 2072–2076 (1976).

    ADS  CAS  Article  Google Scholar 

  12. 11

    Guerineau, M., Grandchamp, C. & Slonimski, P. P. Proc. natn. Acad. Sci. U.S.A. 73, 3030–3034 (1976).

    ADS  CAS  Article  Google Scholar 

  13. 12

    Livingston, D. M. & Klein, H. L. J. Bact. 129, 472–481 (1977).

    CAS  PubMed  Google Scholar 

  14. 13

    Livingston, D. M. Genetics 86, 73–84 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. 14

    Cameron, J. R., Philippsen, P. & Davis, R. W. Nucleic Acids Res. 4, 1429–1448 (1977).

    CAS  Article  Google Scholar 

  16. 15

    Bolivar, F., Rodriguez, R. L., Betlach, M. C. & Boyer, H. W. Gene 2, 75–93 (1977).

    CAS  Article  Google Scholar 

  17. 16

    Guerineau, M., Slonimski, P. P. & Avner, P. R. Biochem. biophys. Res. Commun. 61, 462–469 (1974).

    CAS  Article  Google Scholar 

  18. 17

    Jackson, D., Symons, R. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 69, 2904–2909 (1972).

    ADS  CAS  Article  Google Scholar 

  19. 18

    Somers, J. M., Amzallag, A. & Middleton, R. B. J. Bact. 113, 1268–1272 (1973).

    CAS  PubMed  Google Scholar 

  20. 19

    Borck, K., Beggs, J. D., Brammer, W. J., Hopkins, A. S. & Murray, N. E. Molec. gen. Genet. 146, 199–207 (1976).

    CAS  Article  Google Scholar 

  21. 20

    Clewell, D. J. Bact. 110, 667–676 (1972).

    CAS  PubMed  Google Scholar 

  22. 21

    Clewell, D. D. & Helinski, D. R. Proc. natn. Acad. Sci. U.S.A. 62, 1159–1166 (1969).

    ADS  CAS  Article  Google Scholar 

  23. 22

    Murray, N. E., Brammar, W. J. & Murray, K. Molec. gen. Genet. 150, 53–61 (1977).

    CAS  Article  Google Scholar 

  24. 23

    Lederberg, E. M. & Cohen, S. N. J. Bact. 119, 1072–1074 (1974).

    CAS  PubMed  Google Scholar 

  25. 24

    Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    ADS  CAS  Article  Google Scholar 

  26. 25

    Maniatis, T., Jeffrey, A. & Kleid, D. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

    ADS  CAS  Article  Google Scholar 

  27. 26

    Barnes, W. M. Science 195, 393–394 (1977).

    ADS  CAS  Article  Google Scholar 

  28. 27

    Davidson, E. H., Hough, B. R., Amenson, C. S. & Britten, R. J. J. molec. Biol. 77, 1–23 (1973).

    CAS  Article  Google Scholar 

  29. 28

    Lobban, P. E. & Kaiser, A. D. J. molec. Biol. 78, 453–471 (1973).

    CAS  Article  Google Scholar 

  30. 29

    Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 863–877 (1976).

    CAS  Article  Google Scholar 

  31. 30

    Spizizen, J. Proc. natn. Acad. Sci. U.S.A. 44, 1072–1078 (1958).

    ADS  CAS  Article  Google Scholar 

  32. 31

    Burgess, R. R. J. biol. Chem. 244, 6160–6167 (1969).

    CAS  PubMed  Google Scholar 

  33. 32

    Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    CAS  Article  Google Scholar 

  34. 33

    van Solingen, P. & van der Plaat, J. B. J. Bact. 130, 946–947 (1977).

    CAS  PubMed  Google Scholar 

  35. 34

    Cryer, D. R., Eccleshall, R. & Marmur, J. Methods in Cell Biology XII, 39–44 (1975).

    Article  Google Scholar 

  36. 35

    Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1977).

    CAS  Article  Google Scholar 

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Beggs, J. Transformation of yeast by a replicating hybrid plasmid. Nature 275, 104–109 (1978). https://doi.org/10.1038/275104a0

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