Abstract
In the lacI gene of Escherichia coli spontaneous base substitution hotspots occur at 5-methylcytosine residues. The hotspots disappear when the respective cytosines are not methylated. We suggest that the hotspots may result from the spontaneous deamination of 5-methylcytosine to thymine, which is not excised by the enzyme DNA-uracil glycosidase.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Benzer, S. Proc. natn. Acad. Sci. U.S.A. 47, 403–416 (1961).
Farabaugh, P. J., Schmeissner, U., Hofer, M. & Miller, J. H. J. molec. Biol. (in the press).
Coulondre, C. & Miller, J. H. J. molec. Biol. 117, 577–606 (1977).
Coulondre, C. & Miller, J. H. J. molec. Biol. 117, 525–575 (1977).
Miller, J. H., Coulondre, C. & Farabaugh, P. J. Nature 274, 770–775 (1978).
Steege, D. A. Proc. natn. Acad. Sci. U.S.A. 74, 4163ndash;4167 (1977).
Farabaugh, P. J. Nature 274, 765–769 (1978).
Gold, M., Gefter, R., Hausmann, R. & Hurwitz, J. in Macromolecular Metabolism 5–28 (Little Brown & Co., Boston, 1966).
Boyer, H. W., Chow, L. T., Dugaiczyk, A., Hedgpeth, J. & Goodman, H. M. Nature new biol. 244, 40–48 (1973).
Lederberg, S. J. molec. Biol. 17, 293–297 (1966).
Calos, M. Nature 274, 762–765 (1978).
Miller, J. H., Ganem, D., Lu, P. & Schmitz, A. J. molec. Biol. 109, 245–302 (1977).
Yanofsky, C., Ito, J. & Horn, V. Cold Spring Harb. Symp. quant. Biol. 31, 151–162 (1966).
Osborn, M., Person, S., Phillips, S. & Funk, F. J. molec. Biol. 26, 437–447 (1967).
Drake, J. W. & Baltz, R. H. A. Rev. Biochem. 45, 11–37 (1976).
Jencks, W. P. & Regenstein, J. CRC Handbook of Biochemistry 5187–5226 (1976).
Shapiro, R. & Klein, R. S. Biochemistry 5, 2358–2362 (1966).
Lindahl, T., Ljungquist, S., Siegert, W., Nyberg, B. & Sperens, B. J. biol. Chem. 252, 3286–3294 (1977).
Baltz, R. H., Bingham, P. M. & Drake, J. W. Proc. natn. Acad. Sci. U.S.A. 73, 1269–1273 (1976).
Duncan, B. K., Rockstroh, P. A. & Warner, H. R. Fedn Proc. 35, 1493 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coulondre, C., Miller, J., Farabaugh, P. et al. Molecular basis of base substitution hotspots in Escherichia coli. Nature 274, 775–780 (1978). https://doi.org/10.1038/274775a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/274775a0
This article is cited by
-
MBD4 loss results in global reactivation of promoters and retroelements with low methylated CpG density
Journal of Experimental & Clinical Cancer Research (2023)
-
Genetic diversity, linkage disequilibrium, and population structure of tetraploid wheat landraces originating from Europe and Asia
BMC Genomics (2023)
-
An antisense Alu transposon insertion/deletion polymorphism of ALDH1A1 may functionally associate with Parkinson’s disease
BMC Geriatrics (2022)
-
Transposable elements maintain genome-wide heterozygosity in inbred populations
Nature Communications (2022)
-
Unusual clinical manifestations and predominant stopgain ATM gene variants in a single centre cohort of ataxia telangiectasia from North India
Scientific Reports (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.