Article | Published:

A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells

Nature volume 274, pages 445449 (03 August 1978) | Download Citation

Subjects

Abstract

During the acquisition of aggregation competence a new antigen appears on the surface of Dictyostelium cells. Univalent antibody fragments (Fab) against this antigen render the cells unable to form the specific type of cell adhesion which is characteristic of aggregating cells. This membrane constituent has been purified and identified as a concanavalin A-binding glycoprotein present at about 2 × 105 copies per cell.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    & in Cell and Tissue Interactions (eds Lash, J. W. & Burger, M. M.) 155–172 (Raven, New York, 1977).

  2. 2.

    , & Devl Biol. 56, 343–355 (1977).

  3. 3.

    , , & Proc. natn. Acad. Sci. U.S.A. 74, 4449–4452 (1977).

  4. 4.

    & Devl Biol. 43, 264–276 (1975).

  5. 5.

    Receptors Recognition 3, 47–100 (1977).

  6. 6.

    , & Proc. natn. Acad. Sci. U.S.A. 70, 2309–2312 (1973).

  7. 7.

    , & Nature 264, 66–68 (1976).

  8. 8.

    Expl Cell Res. 22, 455–475 (1961).

  9. 9.

    , , , & Proc. natn. Acad. Sci. U.S.A. 73, 577–581 (1976).

  10. 10.

    & Proc. natn. Acad. Sci. U.S.A. 73, 3594–3598 (1976).

  11. 11.

    et al. Surface Membrane Receptors (eds Bradshaw, R. A., Frazier, W. A., Merrell, R. C., Gottlieb, D. I. & Hogue-Angeletti, R. A.) 109–131 (Plenum, New York, 1976).

  12. 12.

    , & Biochim. biophys. Acta 457, 385–416 (1976).

  13. 13.

    , , , & Expl Cell Res. 63, 147–158 (1970).

  14. 14.

    , & J. Cell Biol. 56, 647–658 (1973).

  15. 15.

    Expl Cell Res. 25, 535–554 (1961).

  16. 16.

    , , & Proc. natn. Acad. Sci. U.S.A. 70, 3150–3154 (1973).

  17. 17.

    & FEBS Lett. 56, 46–49 (1975).

  18. 18.

    , , & J. biol. Chem. 252, 6841–6845 (1977).

  19. 19.

    & Naturwissenschaften 55, 656 (1968).

  20. 20.

    & J. Membrane Biol. 5, 215–224 (1971).

  21. 21.

    & Cell Diff. 6, 339–346 (1977).

  22. 22.

    , & Expl Cell Res. 110, 267–276 (1977).

  23. 23.

    , , & Proc. natn. Acad. Sci. U.S.A. 70, 2554–2557 (1973).

  24. 24.

    , , & Expl Cell Res. 95, 136–142 (1975).

  25. 25.

    , , , & J. molec. Biol. 100, 157–178 (1976).

  26. 26.

    , , & Proc. natn. Acad. Sci. U.S.A. 72, 3541–3545 (1975).

  27. 27.

    , & Nature 263, 426–427 (1976).

  28. 28.

    , & Devl Biol. 61, 202–213 (1977).

  29. 29.

    & J. molec. Biol. 120, 265–279 (1978).

  30. 30.

    & J. Cell Biol. 74, 264–273 (1977).

  31. 31.

    , & Cell 8, 391–396 (1976).

  32. 32.

    , , , & Miami Winter Symp. 7, 49–66 (1974).

  33. 33.

    , , & Eur. J. Biochem. 48, 89–101 (1974).

  34. 34.

    thesis, Tübingen Univ., 1973.

  35. 35.

    & J. immun. Meth. 2, 49–57 (1972).

  36. 36.

    Nature 227, 680–682 (1970).

  37. 37.

    & Analyt. Biochem. 63, 414–417 (1975).

Download references

Author information

Affiliations

  1. Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland

    • Kurt Müller
    •  & Günther Gerisch

Authors

  1. Search for Kurt Müller in:

  2. Search for Günther Gerisch in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/274445a0

Further reading

  • Biological soliton in multicellular movement

    • Hidekazu Kuwayama
    •  & Shuji Ishida

    Scientific Reports (2013)

  • The origins of the molecular era of adhesion research

    • Alan Rick Horwitz

    Nature Reviews Molecular Cell Biology (2012)

  • The genome of the social amoeba Dictyostelium discoideum

    • L. Eichinger
    • , J. A. Pachebat
    • , G. Glöckner
    • , M.-A. Rajandream
    • , R. Sucgang
    • , M. Berriman
    • , J. Song
    • , R. Olsen
    • , K. Szafranski
    • , Q. Xu
    • , B. Tunggal
    • , S. Kummerfeld
    • , M. Madera
    • , B. A. Konfortov
    • , F. Rivero
    • , A. T. Bankier
    • , R. Lehmann
    • , N. Hamlin
    • , R. Davies
    • , P. Gaudet
    • , P. Fey
    • , K. Pilcher
    • , G. Chen
    • , D. Saunders
    • , E. Sodergren
    • , P. Davis
    • , A. Kerhornou
    • , X. Nie
    • , N. Hall
    • , C. Anjard
    • , L. Hemphill
    • , N. Bason
    • , P. Farbrother
    • , B. Desany
    • , E. Just
    • , T. Morio
    • , R. Rost
    • , C. Churcher
    • , J. Cooper
    • , S. Haydock
    • , N. van Driessche
    • , A. Cronin
    • , I. Goodhead
    • , D. Muzny
    • , T. Mourier
    • , A. Pain
    • , M. Lu
    • , D. Harper
    • , R. Lindsay
    • , H. Hauser
    • , K. James
    • , M. Quiles
    • , M. Madan Babu
    • , T. Saito
    • , C. Buchrieser
    • , A. Wardroper
    • , M. Felder
    • , M. Thangavelu
    • , D. Johnson
    • , A. Knights
    • , H. Loulseged
    • , K. Mungall
    • , K. Oliver
    • , C. Price
    • , M. A. Quail
    • , H. Urushihara
    • , J. Hernandez
    • , E. Rabbinowitsch
    • , D. Steffen
    • , M. Sanders
    • , J. Ma
    • , Y. Kohara
    • , S. Sharp
    • , M. Simmonds
    • , S. Spiegler
    • , A. Tivey
    • , S. Sugano
    • , B. White
    • , D. Walker
    • , J. Woodward
    • , T. Winckler
    • , Y. Tanaka
    • , G. Shaulsky
    • , M. Schleicher
    • , G. Weinstock
    • , A. Rosenthal
    • , E. C. Cox
    • , R. L. Chisholm
    • , R. Gibbs
    • , W. F. Loomis
    • , M. Platzer
    • , R. R. Kay
    • , J. Williams
    • , P. H. Dear
    • , A. A. Noegel
    • , B. Barrell
    •  & A. Kuspa

    Nature (2005)

  • Analysis of gene function inDictyostelium

    • A. Kuspa
    • , T. Dingermann
    •  & W. Nellen

    Experientia (1995)

  • Cell adhesion in the life cycle ofDictyostelium

    • S. Bozzaro
    •  & E. Ponte

    Experientia (1995)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.