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Structure and processing of yeast precursor tRNAs containing intervening sequences

Nature volume 274, pages 438445 (03 August 1978) | Download Citation

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Abstract

We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

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References

  1. 1.

    et al. Proc. natn. Acad. Sci. U.S.A. 75, 725–729 (1978).

  2. 2.

    & Cell 12, 1097–1108 (1977).

  3. 3.

    , & Nature 270, 314–319 (1977).

  4. 4.

    & Proc. natn. Acad. Sci. U.S.A. 74, 5652–5656 (1977).

  5. 5.

    , , , & Proc. natn. Acad. Sci. U.S.A. 74, 3686–3690 (1977).

  6. 6.

    , & Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1977).

  7. 7.

    , , & Cell (in the press).

  8. 8.

    , , & Cell 12, 1–8 (1977).

  9. 9.

    Cell 12, 9–21 (1977).

  10. 10.

    , & Proc. natn. Acad. Sci. U.S.A. 74, 5433–5457 (1977).

  11. 11.

    , , , & Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).

  12. 12.

    et al. Nature 267, 639–641 (1977).

  13. 13.

    Nucleic Acids Res. 4, 2831–2841 (1977).

  14. 14.

    , , , & Cell 14, 221–236 (1978).

  15. 15.

    , , , & Proc. natn. Acad. Sci. U.S.A. 75, 1309–1313 (1978).

  16. 16.

    & J. Bact. 96, 1664–1671 (1968).

  17. 17.

    , & J. Bact. 99, 807–814 (1969).

  18. 18.

    & Proc. natn. Acad. Sci. U.S.A. 71, 2658–2662 (1974).

  19. 19.

    , , , & Virology 65, 248–259 (1975).

  20. 20.

    , & J. molec. Biol. 13, 373–398 (1965).

  21. 21.

    , & Analyt. Biochem. 72, 433–446 (1976).

  22. 22.

    in Laboratory Techniques in Biochemistry and Molecular Biology (eds Work, T. S. & Work, E.) (Elsevier, New York).

  23. 23.

    , & Science 153, 531–534 (1966).

  24. 24.

    et al. Nature 262, 757–761 (1976).

  25. 25.

    & Nucleic Acids Res. 2, 865–871 (1975).

  26. 26.

    Nucleic Acids Res. 2, 1073–1082 (1975).

  27. 27.

    , & FEBS Lett. 50, 185–189 (1975).

  28. 28.

    , & Cell 8, 581–594 (1976).

  29. 29.

    et al. Nature new Biol. 246, 6–11 (1973).

  30. 30.

    et al. J. biol. Chem. 252, 3987–3994 (1977).

  31. 31.

    , , & Proc. natn. Acad. Sci. U.S.A. 74, 3872–3876 (1976).

  32. 32.

    , , & Proc. natn. Acad. Sci. U.S.A. 74, 1965–1968 (1977).

  33. 33.

    , , & Cell 6, 395–405 (1975).

  34. 34.

    , , & Cell 6, 407–413 (1975).

  35. 35.

    Meth. Enzym. 17A, 59–78 (1970).

  36. 36.

    J. biol. Chem. 248, 3860–3875 (1973).

  37. 37.

    , & J. biol. Chem. 244, 924–928 (1969).

  38. 38.

    & Biochemistry 6, 1818–1827 (1967).

  39. 39.

    , , & J. molec. Biol. 29, 457–471 (1967).

  40. 40.

    Biochem. biophys. Res. Commun. 23, 641–646 (1966).

  41. 41.

    , , & J. Bact. 125, 25–32 (1974).

  42. 42.

    in Methods in Cell Biology, Vol. 6, (ed. Prescott, D. M.) 77–97 (Academic, New York, 1975).

  43. 43.

    & Biochim. Biophys. Acta 47, 580–586 (1961).

  44. 44.

    Eur. J. Biochem. 41, 197–202 (1974).

  45. 45.

    Eur. J. Biochem. 33, 192–200 (1973).

  46. 46.

    Biochim. Biophys. Acta 114, 158–168 (1966).

  47. 47.

    , & , Cell, 14, 211–219 (1978).

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  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143

    • Patricia Z. O'Farrell
    • , Barbara Cordell
    • , Pablo Valenzuela
    • , William J. Rutter
    •  & Howard M. Goodman

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https://doi.org/10.1038/274438a0

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