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Structure and processing of yeast precursor tRNAs containing intervening sequences

Nature volume 274, pages 438–445 (1978)Cite this article

Abstract

We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

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References

  1. Tilghman, S. M. et al. Proc. natn. Acad. Sci. U.S.A. 75, 725–729 (1978).

    Article  ADS  CAS  Google Scholar 

  2. Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

    Article  CAS  Google Scholar 

  3. Breathnach, R., Mandel, J. L. & Chambon, P. Nature 270, 314–319 (1977).

    Article  ADS  CAS  Google Scholar 

  4. Brack, C. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 74, 5652–5656 (1977).

    Article  ADS  CAS  Google Scholar 

  5. Aloni, Y., Dhar, R., Laub, O., Horowitz, M. & Khoury, G. Proc. natn. Acad. Sci. U.S.A. 74, 3686–3690 (1977).

    Article  ADS  CAS  Google Scholar 

  6. Berget, S. M., Moore, C. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Cordell, B., Weiss, S. R., Varmus, H. E. & Bishop, J. M. Cell (in the press).

  8. Chow, L. T., Gelinas, R. E., Broker, T. R. & Roberts, R. J. Cell 12, 1–8 (1977).

    Article  CAS  Google Scholar 

  9. Klessig, D. F. Cell 12, 9–21 (1977).

    Article  CAS  Google Scholar 

  10. Goodman, H. M., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5433–5457 (1977).

    ADS  Google Scholar 

  11. Valenzuela, P., Venegas, A., Weinberg, F., Bishop, R. & Rutter, W. J. Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).

    Article  ADS  CAS  Google Scholar 

  12. Olson, M. V. et al. Nature 267, 639–641 (1977).

    Article  ADS  CAS  Google Scholar 

  13. Feldman, H. Nucleic Acids Res. 4, 2831–2841 (1977).

    Article  CAS  Google Scholar 

  14. Knapp, G., Beckmann, J. S., Johnson, P. F., Fuhrman, S. A. & Abelson, J. Cell 14, 221–236 (1978).

    Article  CAS  Google Scholar 

  15. Tilghman, S. M., Curtiss, P. J., Tiemeier, D. C., Leder, P. & Weissman, C. Proc. natn. Acad. Sci. U.S.A. 75, 1309–1313 (1978).

    Article  ADS  CAS  Google Scholar 

  16. Hartwell, L. H. & McLaughlin, C. S. J. Bact. 96, 1664–1671 (1968).

    CAS  Google Scholar 

  17. Hutchinson, H. T., Hartwell, L. H. & McLaughlin, C. S. J. Bact. 99, 807–814 (1969).

    Google Scholar 

  18. Shiokawa, K. & Pogo, A. O. Proc. natn. Acad. Sci. U.S.A. 71, 2658–2662 (1974).

    Article  ADS  CAS  Google Scholar 

  19. Taylor, J. M., Cordell-Stewart, B., Rohde, W., Goodman, H. M. & Bishop, J. M. Virology 65, 248–259 (1975).

    Article  CAS  Google Scholar 

  20. Sanger, F., Brownlee, G. G. & Barrell, B. G. J. molec. Biol. 13, 373–398 (1965).

    Article  CAS  Google Scholar 

  21. Volckaert, G., Jou, W. M. & Fiers, W. Analyt. Biochem. 72, 433–446 (1976).

    Article  CAS  Google Scholar 

  22. Brownlee, G. G. in Laboratory Techniques in Biochemistry and Molecular Biology (eds Work, T. S. & Work, E.) (Elsevier, New York).

  23. Madison, J. T., Everett, G. A. & Kung, H. Science 153, 531–534 (1966).

    Article  ADS  CAS  Google Scholar 

  24. Piper, P. W. et al. Nature 262, 757–761 (1976).

    Article  ADS  CAS  Google Scholar 

  25. Harada, F. & Dahlberg, J. E. Nucleic Acids Res. 2, 865–871 (1975).

    Article  CAS  Google Scholar 

  26. Tal, J. Nucleic Acids Res. 2, 1073–1082 (1975).

    Article  CAS  Google Scholar 

  27. Fradin, A., Gruhl, H. & Feldman, H. FEBS Lett. 50, 185–189 (1975).

    Article  CAS  Google Scholar 

  28. Schedl, P., Roberts, J. & Primakoff, P. Cell 8, 581–594 (1976).

    Article  CAS  Google Scholar 

  29. Guthrie, C. et al. Nature new Biol. 246, 6–11 (1973).

    Article  CAS  Google Scholar 

  30. Sugino, A. et al. J. biol. Chem. 252, 3987–3994 (1977).

    CAS  PubMed  Google Scholar 

  31. Gellert, M., Mizuichi, K., O'Dea, M. H. & Nash, H. A. Proc. natn. Acad. Sci. U.S.A. 74, 3872–3876 (1976).

    Article  ADS  Google Scholar 

  32. Marians, K. J., Ikeda, J., Schlagman, S. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 74, 1965–1968 (1977).

    Article  ADS  CAS  Google Scholar 

  33. Gillum, A. M., Urquhart, N., Smith, M. & RajBhandary, U. L. Cell 6, 395–405 (1975).

    Article  CAS  Google Scholar 

  34. Gillum, A. M., Roe, B. A., Anandaraj, M. P. J. S. & RajBhandary, U. L. Cell 6, 407–413 (1975).

    Article  CAS  Google Scholar 

  35. Fink, G. R. Meth. Enzym. 17 A, 59–78 (1970).

    Article  Google Scholar 

  36. Rubin, G. M. J. biol. Chem. 248, 3860–3875 (1973).

    CAS  PubMed  Google Scholar 

  37. Price, P. A., Moore, S. & Stein, W. H. J. biol. Chem. 244, 924–928 (1969).

    CAS  PubMed  Google Scholar 

  38. Peacock, A. C. & Dingman, C. W. Biochemistry 6, 1818–1827 (1967).

    Article  CAS  Google Scholar 

  39. Landy, A., Abelson, J., Goodman, H. M. & Smith, J. D. J. molec. Biol. 29, 457–471 (1967).

    Article  CAS  Google Scholar 

  40. Denhardt, D. Biochem. biophys. Res. Commun. 23, 641–646 (1966).

    Article  CAS  Google Scholar 

  41. Thonart, P., Bechet, J., Hilger, F. & Burny, A. J. Bact. 125, 25–32 (1974).

    Google Scholar 

  42. Duffus, J. H. in Methods in Cell Biology, Vol. 6, (ed. Prescott, D. M.) 77–97 (Academic, New York, 1975).

    Google Scholar 

  43. Hunter, M. J. & Commerford, S. L. Biochim. Biophys. Acta 47, 580–586 (1961).

    Article  CAS  Google Scholar 

  44. Rubin, G. M. Eur. J. Biochem. 41, 197–202 (1974).

    Article  CAS  Google Scholar 

  45. Vogt, V. M. Eur. J. Biochem. 33, 192–200 (1973).

    Article  CAS  Google Scholar 

  46. Ando, T. Biochim. Biophys. Acta 114, 158–168 (1966).

    Article  CAS  Google Scholar 

  47. Hopper, A. K., Banks, F. & Evangelidis, Cell, 14, 211–219 (1978).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143

    Patricia Z. O'Farrell, Barbara Cordell, Pablo Valenzuela, William J. Rutter & Howard M. Goodman

Authors
  1. Patricia Z. O'Farrell
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  2. Barbara Cordell
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  3. Pablo Valenzuela
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  4. William J. Rutter
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  5. Howard M. Goodman
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O'Farrell, P., Cordell, B., Valenzuela, P. et al. Structure and processing of yeast precursor tRNAs containing intervening sequences. Nature 274, 438–445 (1978). https://doi.org/10.1038/274438a0

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