Abstract
EcoRI fragments of the natural ovalbumin gene were cloned and studied by hybridisation with mature ovalbumin mRNA, electron microscopy, restriction enzyme mapping and limited sequence analysis. The structural gene sequences coding for ovalbumin were found to be separated into eight sequentially orientated pieces by seven intervening sequences of various lengths.
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References
Botchan, T., Topp, W. & Sambrook, J. Cell 9, 269–287 (1976).
Watson, J. D. in Molecular Biology of the Gene (W. A. Benjamin, Menlo Park, 1976).
Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).
Tilghman, S. M. et al. Proc. natn. Acad. Sci. U.S.A. 74, 4406–4409 (1977).
Brack, C. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 74, 5652–5656 (1977).
Glover, D. M. & Hogness, D. S. Cell 10, 167–176 (1977).
White, R. L. & Hogness, D. S. Cell 10, 177–192 (1977).
Wellauer, P. K. & Dawid, I. B. Cell 10, 193–212 (1977).
Goodman, H. M., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5453–5457 (1977).
Valenzuela, P., Venegas, A., Weinberg, F., Bishop, R. & Rutter, W. J. Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).
Breathnach, R., Mandel, J. L. & Chambon, P. Nature 270, 314–319 (1977).
Doel, M. T., Houghton, M., Cook, E. A. & Carey, N. H. Nucleic Acids Res. 4, 3701–3713 (1977).
Lai, E. C., Woo, S. L. C., Dugaiczyk, A., Catterall, J. F. & O'Malley, B. W. Proc. natn. Acad. Sci. U.S.A. 75, 2205–2209 (1978).
Dugaiczyk, A. et al. in Int. Symp. genet. Engng, Milan, March, 1978 (in the press).
Weinstock, R., Sweet, R., Weise, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).
McReynolds, L. et al. Nature 273, 723–728 (1978).
McReynolds, L. A., Catterall, J. F. & O'Malley, B. W. Gene 2, 217–231 (1977).
Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).
Hardies, S. C. & Wells, R. D. Proc. natn. Acad. Sci. U.S.A. 9, 3117–3121 (1976).
Leder, P., Tiemeier, D. & Enquist, L. Science 196, 175–177 (1977).
Tiemeier, D. C., Tilghman, S. M. & Leder, P. Gene 2, 173–191 (1977).
Benton, W. D. & Davies, R. W. Science 196, 180–182 (1977).
Woo, S. L. C. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Dugaiczyk, A., Boyer, H. W. & Goodman, H. M. J. molec. Biol. 96, 171–184 (1975).
Southern, E. M. J. molec. Biol. 98, 503–517 (1977).
Garapin, A. C. et al. in Int. Symp. genet. Engng, Milan, March 1978 (in the press).
Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
O'Malley, B. W., McGuire, W. L., Kohler, P. K. & Korenman, S. G. in Recent Progress in Hormone Research, Vol. 13, 105–160 (Academic, New York, 1969).
O'Malley, B. W. & Means, A. R. Science 183, 610–620 (1974).
Schrader, W. T. & O'Malley, B. W. in Receptors and Hormone Action, Vol. 2, 189–224 (Academic, New York, 1978).
O'Malley, B. W., Towle, H. C. & Schwartz, R. J. A. Rev. Genet. 11, 239–275 (1977).
Helling, R. B., Goodman, H. M. & Boyer, H. W. J. Virol. 14, 1235–1244 (1974).
Dingman, C. W., Fisher, M. P. & Kakefuda, T. Biochemistry 11, 1242–1250 (1972).
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Dugaiczyk, A., Woo, S., Lai, E. et al. The natural ovalbumin gene contains seven intervening sequences. Nature 274, 328–333 (1978). https://doi.org/10.1038/274328a0
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DOI: https://doi.org/10.1038/274328a0
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