Letter | Published:

Cytological detection of crossing-over in BUdR substituted meiotic chromosomes using the fluorescent plus Giemsa technique

Nature volume 272, pages 823824 (27 April 1978) | Download Citation

Subjects

Abstract

THE relationship between chiasmata and crossing-over has long been of interest, for if chiasmata are the cytological manifestations of recombination events they represent an easily accessible and reliable source of information on the frequency and distribution of crossing-over throughout the entire genome. Autoradiographic studies on meiotic chromosomes have proved that chiasma formation involves breakage and reunion of homologous, non-sister chromatids1–4. However, the inherent lack of precision in autoradiography has demanded that the stages of meiosis investigated, usually anaphase I or later, are remote from the time of crossing-over, and after homologous chromosome pairs have disjoined. This has precluded a comparison of chiasma distributions with cross-over positions, with one exception. In the grasshopper Stethophyma grossum, the proximal localisation of chiasmata at metaphase I has been shown to correspond perfectly with the exchange positions of autoradiographic label at anaphase I (ref. 4).

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    J. Cell Biol. 25, 57–67 (1965).

  2. 2.

    & Heredity 25, 223–232 (1970).

  3. 3.

    Genetics 65, 593–617 (1970).

  4. 4.

    Chromosoma 34, 367–382 (1971).

  5. 5.

    & Nature 251, 156–158 (1974).

  6. 6.

    & Chromosoma 51, 1–10 (1975).

  7. 7.

    , Chromosoma 58, 325–340 (1976).

  8. 8.

    Recent Advances in Cytology (Churchill, London, 1932).

  9. 9.

    Meiotic Configurations (eds Frankel, R. Grossman, M. Linskens, H. F. & de Zeeuw, D.) (Springer, Berlin, Heidelberg and New York, 1975).

Download references

Author information

Affiliations

  1. Department of Genetics, University of Birmingham, Birmingham, UK

    • C. TEASE

Authors

  1. Search for C. TEASE in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/272823a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.