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Immunoglobulin light-chain structural gene sequences cloned in a bacterial plasmid

Nature volume 271, pages 582–585 (1978)Cite this article

Abstract

IMMUNOGLOBULIN light-chain mRNA molecules and their cDNA transcripts have served as molecular probes in hybridisation experiments designed to quantify the number of immunoglobulin genes1–13 and to characterise the arrangement of these genes in the mammalian genome14,15. In other studies these mRNAs and cDNAs have been used as substrates for nucleotide sequence analyses to define the structural gene encoding immunoglobulin light chains16,17. Unfortunately, two central technical problems have developed during these studies. First, the immunoglobulin mRNA cannot be obtained in amounts sufficient for many experiments. For example, determination of the complete nucleotide sequence of this RNA remains difficult without more material. Second, immunoglobulin mRNA cannot be obtained as a homogeneous RNA completely free of other RNA species. These impurities have probably interfered with several hybridisation studies. In order to obtain a substrate for nucleotide sequence analysis as well as to provide a pure hybridisation probe we have cloned the DNA (cDNA) complementary to the immunoglobulin light-chain (kappa) mRNA from the mouse myeloma MOPC-149. The plasmid pCRl-κ40 is shown here to contain sequences corresponding to 700 bases of the immunoglobulin mRNA.

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Author notes

  1. MARSHALL H. EDGELL

    Present address: Department of Bacteriology and Immunology, Curriculum in Genetics, University of North Carolina, Chapel Hill, North Carolina, 27514

Authors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20014

    J. G. SEIDMAN, MARSHALL H. EDGELL & PHILIP LEDER

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  3. PHILIP LEDER
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SEIDMAN, J., EDGELL, M. & LEDER, P. Immunoglobulin light-chain structural gene sequences cloned in a bacterial plasmid. Nature 271, 582–585 (1978). https://doi.org/10.1038/271582a0

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