Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A Rhizobium mutant incapable of nodulation and normal polysaccharide secretion

Abstract

THE surface carbohydrates of Rhizobium, including both the lipopolysaccharide and the extracellular polysaccharide, are thought to participate in the infection and nodulation of legumes by these Gram-negative bacteria1–3. We report here the isolation of a mutant of Rhizobium leguminosarum which produces diminished amounts of extracellular polysaccharide (that is, the carbohydrate material present in cell-free filtrates of bacterial cultures) and which fails to nodulate pea, its normal host.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Dazzo, F. B. & Hubbel, D. H. Appl. Microbiol. 30, 1017–1033 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Wolpert, J. S. & Albersheim, P. Biochem. biophys. Res. Commun. 70, 729–737 (1976).

    Article  CAS  Google Scholar 

  3. Planque, K. & Kijne, J. W. FEBS Lett. 73, 64–66 (1976).

    Google Scholar 

  4. Bergersen, F. Aust. J. biol. Sci. 14, 349 (1961).

    Article  CAS  Google Scholar 

  5. Vincent, J. M. A Manual for the Practical Study of the Root-Nodule Bacteria (Blackwell, Oxford, 1970).

    Google Scholar 

  6. Dische, Z. Meth. Carbohydrate Chem. 1, 478–512 (1962).

    CAS  Google Scholar 

  7. Fahraeus, G. J. gen. Microb. 16, 374–381 (1957).

    CAS  Google Scholar 

  8. Weidel, W. A. Rev. Microbiol. 12, 27–48 (1958).

    Article  CAS  Google Scholar 

  9. Humphrey, B. & Vincent, J. M. J. gen. Microbiol. 59, 411–425 (1969).

    Article  CAS  Google Scholar 

  10. Humphrey, B. & Vincent, J. M. J. gen. Microbiol. 41, 109–118 (1965).

    Article  CAS  Google Scholar 

  11. Crowle, A. J. Immunodiffusion (Academic, New York, 1973).

    Google Scholar 

  12. Albersheim, P., Nevins, D. J., English, P. D. & Karr, A. Carbohydrate Res. 5, 340–345 (1967).

    Article  CAS  Google Scholar 

  13. Weissbach, A. & Hurwitz, J. J. biol. Chem. 234, 705–709 (1958).

    Google Scholar 

  14. Blumenkrantz, N. & Asboe-Hansen, G. Analyt. Biochem. 54, 484–489 (1973).

    Article  CAS  Google Scholar 

  15. Lüderitz, O., Staub, A. M. & Westphal, O. Bact. Rev. 30, 192–255 (1966).

    PubMed  Google Scholar 

  16. Mäkelä, H. & Stocker, B. A. Rev. Genet. 3, 291 (1969).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

SANDERS, R., CARLSON, R. & ALBERSHEIM, P. A Rhizobium mutant incapable of nodulation and normal polysaccharide secretion. Nature 271, 240–242 (1978). https://doi.org/10.1038/271240a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/271240a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing