Abstract
ANEUPLOIDY or heteroploidy and hyperploidy have been observed in tumours of experimental animals and of humans and seem to be a feature of most malignant cells1–9. DNA contents seem to correlate with karyotype in these changes or deviations from euploidy. Most tumours are hyperploid and higher in DNA content2,8,9. A rapid and reliable method of analysis of DNA content distribution of a cell population has recently become available with the development of flow microfluorometry (FMF)10. We report here the use of FMF analysis to measure DNA content of clones isolated from a fibrosarcoma. All the clones examined had a higher DNA content than did either the normal cells tested or the original fibrosarcoma. There is thus a variability of DNA content in fibrosarcoma cells. This suggests that variability, as well as increase in DNA content in malignant cells may relate to their neoplastic development.
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References
Foulds, L. Neoplastic Developments 1, 439 (Academic, London & New York. 1969).
German, J. (ed.) Chromosomes and Cancer 756 (Wiley, New York. 1974).
Hauschka, T. S. Cancer Res. 21, 957–974 (1961).
Hsu, T. C. Int. Rev. Cyt. 12, 69–161 (1961).
Makino, S. Int. Rev. Cyt. 6, 26–84 (1957).
Mitelman, F. Hereditas 70, 1–14 (1972).
Nowel, P. C. Science 194, 23–28 (1976).
Atkin, N. B., Richards, B. M. & Ross, A. J. Br. J. Cancer 13, 773–787 (1959).
Stich, H. F. J. natn. Cancer Inst. 24, 1283–1297 (1960).
Steinkamp, J. A. et al. Rev. Scient. Instrum. 44, 1031–1310 (1973).
Suzuki, N. & Okada, S. Mutat. Res. 34, 489–506 (1976).
Barlogie, B. et al. Cancer Res. 36, 1176–1181 (1976).
Crissman, H. A. & Tobey, R. A. Science 184, 1297–1298 (1974).
Grdina, D. J., Linde, S. & Mason, K. Br. J. Radial. (in the press).
Kraemer, P. M., Petersen, D. F. & Van Dilla, M. A. Science 174, 714–717 (1971).
Kraemer, P. M., Deaven, L. L., Crissman, H. A. & Van Dilla, M. A. Adv. cell. molec. Biol. 2, 47–108 (1972).
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SUZUKI, N., WITHERS, H. & LEE, L. Variability of DNA content of murine fibrosarcoma cells. Nature 269, 531–532 (1977). https://doi.org/10.1038/269531a0
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DOI: https://doi.org/10.1038/269531a0
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