Nucleotide sequences of 5′-terminal ribosome-protected initiation regions from two reovirus messages

Abstract

Sequences for two reovirus ribosome-protected fragments are presented. Comparison with four other reovirus initiation sites reveals only two common features: the 5′-terminal sequence m7GpppGmCUA, and (located 15–33 nucleotides from the cap) the sequence AUGG. Both the cap and the AUG codon are included in the 40S-ribosome protected region from all six reovirus messages.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).

    ADS  CAS  Article  Google Scholar 

  2. 2

    Steitz, J. A. & Jakes, K. Proc. natn. Acad. Sci. U.S.A. 72, 4734–4738 (1975).

    ADS  CAS  Article  Google Scholar 

  3. 3

    Dasgupta, R., Shih, D. S., Saris, C. & Kaesberg, P. Nature 256, 624–628 (1975).

    ADS  CAS  Article  Google Scholar 

  4. 4

    Baralle, F. E. Cell 10, 549–558 (1977).

    CAS  Article  Google Scholar 

  5. 5

    Efstratiadis, A., Kafalos, F. C. & Manialis, T. Cell 10, 571–585 (1977).

    CAS  Article  Google Scholar 

  6. 6

    Kozak, M. & Shatkin, A. J. J. molec. Biol. 112, 75–96 (1977).

    CAS  Article  Google Scholar 

  7. 7

    Kozak, M. & Shatkin, A. J. J. biol. Chem.(in the press).

  8. 8

    Shatkin, A. J. Cell 9, 645–653 (1976).

    CAS  Article  Google Scholar 

  9. 9

    Banerjee, A. K. & Shatkin, A. J. J. Virol. 6, 1–11 (1970).

    CAS  Article  Google Scholar 

  10. 10

    Kozak, M. & Shatkin, A. J. J. biol. Chem. 251, 4259–4266 (1976).

    CAS  PubMed  Google Scholar 

  11. 11

    Pieczenik, G., Model, P. & Robertson, H. D. J. molec. Biol. 90, 191–214 (1974).

    CAS  Article  Google Scholar 

  12. 12

    Barrell, B. G. in Procedures in Nucleic Acid Research (eds Cantoni. G. L. & Davies. D. R.) 2, 751–779 (Harper and Row, New York, 1971).

    Google Scholar 

  13. 13

    Hains, J. A., Reese, C. B. & Todd, A. R. J. chem. Soc. 5281 (1967).

  14. 14

    Pett, D. M., Vanaman, T. C. & Joklik, W. K. Virology 52, 174–186 (1973).

    CAS  Article  Google Scholar 

  15. 15

    Legon, S. J. molec. Biol. 106, 37–53 (1976).

    CAS  Article  Google Scholar 

  16. 16

    Rhodes, D. P. & Banerjee, A. K. J. Virol. 17, 33–42 (1976).

    CAS  PubMed Central  Google Scholar 

  17. 17

    Yang, N.-S., Manning, R. F. & Gage, L. P. Cell 7, 339–347 (1976).

    CAS  Article  Google Scholar 

  18. 18

    Lockard, R. E. & RajBhandary, U. L. Cell 9, 747–760 (1976).

    CAS  Article  Google Scholar 

  19. 19

    Shine, J. & Dalgarno, L. Biochem. J. 141, 609–615 (1974).

    CAS  Article  Google Scholar 

  20. 20

    Van de Voorde, A., Contreras, R., Rogiers, R. & Fiers, W. Cell 9, 117–120 (1976).

    CAS  Article  Google Scholar 

  21. 21

    Hefti, E., Bishop, D. H. L., Dubin, D. T. & Stollar, V. J. Virol. 17, 149–159 (1976).

    CAS  PubMed Central  Google Scholar 

  22. 22

    Piper, P. W. & Clark, B. F. C. Nature 247, 516–520 (1974).

    ADS  CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kozak, M. Nucleotide sequences of 5′-terminal ribosome-protected initiation regions from two reovirus messages. Nature 269, 390–394 (1977). https://doi.org/10.1038/269390a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.