Nucleotide sequence of Bombyx mori L. tRNA1Gly


THE functional adaptation of the tRNA population in the posterior silk gland of the silkworm Bombyx mori L during growth and secretion of fibroin1 is the result of changes in the predominant isoaccepting tRNA species to accord with the frequency of various codons in fibroin mRNA (refs 2–4). tRNA adaptation to fibroin biosynthesis can be demonstrated by polyacrylamide gel electrophoresis maps. The main glycine codons GGU and GGA of fibroin mRNA (ref. 3) are translated by means of two prevalent iso-tRNAGly species: iso-tRNA1Gly decodes GGU and GGC whereas iso-tRNA2Gly decodes GGA (refs 4–6). The glycine tRNA1, the first sequenced tRNA from an insect6, has 74 nucleotides and relatively few modified residues7: one thymidine, one pseudouridine, one dihydrouridine, one 1-methyl adenine, 1-methyl guanosine, four 5-methyl cytidine and one unusal 2′-O-methyl uridine in the acceptor stem. It carries the expected GCC anticodon4. Comparison with three other homodecoding tRNAGCCGly species reveals a high degree of homology.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Garel, J. P. J. theor. Biol. 43, 211–225 (1974).

    CAS  Article  Google Scholar 

  2. 2

    Garel, J. P. Nature 260, 805–806 (1976).

    CAS  ADS  Article  Google Scholar 

  3. 3

    Suzuki, Y. & Brown, D. D. J. Molec. Biol. 63, 409–429 (1972).

    CAS  Article  Google Scholar 

  4. 4

    Garel, J. P., Hentzen, D. & J. FEBS Lett. 39, 359–363 (1974).

    CAS  Article  Google Scholar 

  5. 5

    Chen, G. S. & Siddiqui, M. A. Q. Arch. Biochem. Biophys. 161. 109–117 (1974).

    CAS  Article  Google Scholar 

  6. 6

    Garel, J. P., Dirheimer, G. & Keith, G. Z. physiol. Chem. 357, 293 (1976).

    Google Scholar 

  7. 7

    Garel, J. P., Hentzen, D., Schlegel, M. & Dirheimer, G. Biochimie 58, 1089–1100 (1976).

    CAS  Article  Google Scholar 

  8. 8

    Garel, J. P., Garber, R. & Siddiqui, M. A. Q. Biochemistry (in the press).

  9. 9

    Chavancy, G., Daillie, J. & Garel, J. P. Biochimie 53, 1187–1194 (1971).

    CAS  Article  Google Scholar 

  10. 10

    Keith, G., Roy, A., Ebel, J. P. & Dirheimer, G. Biochimie 54, 1405–1415 (1972).

    CAS  Article  Google Scholar 

  11. 11

    Rich, A. & RajBhandary, U. L. A. Rev. Biochen. 45, 805–860 (1976).

    CAS  Article  Google Scholar 

  12. 12

    Yoshida, M. Biochem. Biophys. Res. Commun. 50, 779–784 (1973).

    CAS  Article  Google Scholar 

  13. 13

    Marcu, K. B., Mignery, R. E. & Dudock, B. S. Biochemistry 16, 797–806 (1977).

    CAS  Article  Google Scholar 

  14. 14

    Piper, P. W. Eur. J. Biochem. 51, 295–304 (1975).

    CAS  Article  Google Scholar 

  15. 15

    Roe, B. A., Chen, E. Y. & Tsen, H. Y. Biochem. Biophys. Res. Commun. 68, 1339–1347 (1976).

    CAS  Article  Google Scholar 

  16. 16

    Meza, L., Araya, A., Leon, G., Krauskopf, M., Siddiqui, M. A. Q. & Garel, J. P., FEBS Lett. 77, 255–260 (1977).

    CAS  Article  Google Scholar 

  17. 17

    Squires, C. & Carbon, J. Nature new biol. 233, 274–277 (1971).

    CAS  Article  Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

GAREL, J., KEITH, G. Nucleotide sequence of Bombyx mori L. tRNA1Gly. Nature 269, 350–352 (1977).

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.