Abstract
RECOMBINANT DNA techniques have recently enabled bacterial plasmids carrying eukaryotic sequences syn-thesised in vitro from purified mRNAs to be constructed and cloned in Escherichia coli1–10. We show here that a rabbit globin sequence is not transcribed in E. coli when carried by the pCR1 plasmid. However, after transfer into phage λ, the globin sequence is transcribed under λ control. Thus, there is no basic hindrance to the transcription of a specific mammalian sequence in E. coli.
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KOURILSKY, P., GROS, D., ROUGEON, F. et al. Transcription of a mammalian sequence under phage λ control. Nature 267, 637–639 (1977). https://doi.org/10.1038/267637a0
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DOI: https://doi.org/10.1038/267637a0
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