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Active site of bovine factor XI (plasma thromboplastin antecedent)

Naturevolume 266pages729730 (1977) | Download Citation



FACTOR XI (plasma thromboplastin antecedent) is a plasma glycoprotein which participates in the early stages of blood coagulation1. A deficiency of this protein leads to a haemorrhagic disease in which the intrinsic pathway of blood coagulation is partially or completely blocked2. During normal coagulation, factor XI is converted to a serine protease (factor XIa)3, and this enzyme in turn activates factor IX (Christmas factor) by limited proteolysis4. Factor XIa is inhibited by serine protease inhibitors such as diisopropyl phosphorofluoridate, and this inhibitor is bound to a serine residue in the active site of the enzyme3. We recently purified bovine factor XI approximately 28,000-fold in 30% yield. The final preparation was homogeneous as measured by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoelectrophoresis5. It has a molecular weight of 124,000 as determined by sedimentation equilibrium and is composed of two similar or identical polypeptide chains held together by a disulphide bond(s). We present here data showing that bovine factor XI contains an amino acid sequence which is very similar to the active site region of many other serine proteases involved in the coagulation process.

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    Present address: Department of Biochemistry, Niigata University School of Medicine, Niigata, Japan


  1. Departments of Biochemistry and Medicine, University of Washington School of Medicine, Seattle, Washington, 98195

    •  & EARL W. DAVIE


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