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DNA sequences coding for the H2B histone of Psammechinus miliaris

Naturevolume 266pages603607 (1977) | Download Citation

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Abstract

Starting from restriction enzyme cleavage sites of known topologies the DNA sequences coding for two thirds of the H2B histone protein, together with some 3′ extracistronic sequences, have been determined for the sea urchin Psammechinus miliaris. This unambiguously identifies this gene and further reveals its 5′–3′ polarity and accurate map position in a cloned histone DNA repeat unit.

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Affiliations

  1. Institut für Molekularbiologie II der Universität Zürich, Winterthurerstr. 266A, 8057, Zürich, Switzerland

    • M. L. Birnstiel
    •  & W. Schaffner
  2. Department of Microbiology, The Johns Hopkins University, 725 N. Wolfe Street, Baltimore, Maryland, 21205

    • M. L. Birnstiel
    •  & H. O. Smith
  3. MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge, UK

    • W. Schaffner

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https://doi.org/10.1038/266603a0

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