Abstract
FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies1,2. One of the most powerful features of this approach is that, by cloning, one may easily derive cell lines synthesising monoclonal antibodies despite using non-purified immunogens. The multiple components of a heterogeneous population of hybrid cells are resolved by cloning techniques. This feature makes the system a very powerful tool in the study of complex antigenic structures. The established cell lines offer the further advantage of unlimited permanent supply of material, and the possibility of worldwide standardisation.
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GALFRE, G., HOWE, S., MILSTEIN, C. et al. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266, 550–552 (1977). https://doi.org/10.1038/266550a0
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DOI: https://doi.org/10.1038/266550a0
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