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Malignant transformation of rat embryo fibroblasts by herpes simplex virus types 1 and 2 at suboptimal temperature

Nature volume 265, pages 744–746 (1977)Cite this article

Abstract

HERPES SIMPLEX VIRUS (HSV) transforms cultured mammalian cells in three systems, involving (1) ultraviolet-irradiated virus1–4, (2) increased incubation temperature5,6 and (3) temperature-sensitive mutants7,8. Malignant transformation by HSV has been demonstrated for hamster, rat and mouse cells, using three systems4,8–12. We now report a further system, involving the use of a suboptimal temperature. This method is relatively convenient and seems to be applicable to various rat cell lines.

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Authors and Affiliations

  1. Institut für Medizinische Virologie, Univesität Heidelberg, Im Neuenheimer Feld 324, 6900, Heidelberg

    GHOLAMREZA DARAI

  2. Institut für Virusforschung, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 6900, Heidelberg

    RÜDIGER BRAUN, ROLF MANFRED FLÜGEL & KLAUS MUNK

Authors
  1. GHOLAMREZA DARAI
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  2. RÜDIGER BRAUN
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  3. ROLF MANFRED FLÜGEL
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  4. KLAUS MUNK
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DARAI, G., BRAUN, R., FLÜGEL, R. et al. Malignant transformation of rat embryo fibroblasts by herpes simplex virus types 1 and 2 at suboptimal temperature. Nature 265, 744–746 (1977). https://doi.org/10.1038/265744a0

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